Human Spectrin alpha chain, brain, SPTAN1 ELISA Kit | MyBioSource MBS911505 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Spectrin alpha chain, brain, SPTAN1 ELISA Kit

catalog :

MBS911505

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911505

products type :

ELISA Kit

products full name :

Human Spectrin alpha chain, brain, SPTAN1 ELISA Kit

products short name :

spectrin, alpha, non-erythrocytic 1 (alpha-fodrin)

products name syn :

Human Spectrin alpha chain; brain (SPTAN1) ELISA kit; (ALPHA) II-SPECTRIN; FLJ44613; NEAS; non-erythrocytic spectrin alpha; spectrin; alpha; non-erythrocytic 1 (alpha-fodrin)

other names :

spectrin alpha chain, non-erythrocytic 1 isoform 1; Spectrin alpha chain, non-erythrocytic 1; spectrin alpha chain, non-erythrocytic 1; alpha-fodrin; alpha-II spectrin; fodrin alpha chain; spectrin, non-erythroid alpha chain; spectrin, non-erythroid alpha subunit; spectrin, alpha, non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit

products gene name :

SPTAN1

other gene names :

SPTAN1; SPTAN1; NEAS; EIEE5; SPTA2; NEAS; SPTA2

uniprot entry name :

SPTN1_HUMAN

reactivity :

Human

sequence length :

2477

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SPTAN1. No significant cross-reactivity or interference between Human SPTAN1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPTAN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPTAN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPTAN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPTAN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

194595509

ncbi acc num :

NP_001123910.1

ncbi gb acc num :

NM_001130438.2

uniprot acc num :

Q13813

ncbi mol weight :

284,539 Da

ncbi pathways :

Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Axon Guidance Pathway (575211); Axon Guidance Pathway (105688); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Cell-Cell Communication Pathway (477132); Developmental Biology Pathway (575210); Developmental Biology Pathway (477129)

ncbi summary :

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

uniprot summary :

SPTAN1: a cytoskeletal protein which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane. Capable to forming dimers which can further associate to tetramers. Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 9q34.11. Cellular Component: microtubule cytoskeleton; spectrin; membrane; intracellular membrane-bound organelle; fascia adherens; cytosol; Z disc; vesicle; lateral plasma membrane. Molecular Function: calmodulin binding; protein binding; spectrin binding; structural constituent of cytoskeleton; protein heterodimerization activity; calcium ion binding; actin binding. Biological Process: axon guidance; apoptosis; actin filament capping; cell structure disassembly during apoptosis. Disease: Epileptic Encephalopathy, Early Infantile, 5

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage