product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human R-spondin-2, RSPO2 ELISA Kit
catalog :
MBS911461
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS911461
products type :
ELISA Kit
products full name :
Human R-spondin-2, RSPO2 ELISA Kit
products short name :
R-spondin 2 homolog (Xenopus laevis)
products name syn :
Human R-spondin-2 (RSPO2) ELISA kit; CRISTIN2; MGC35555; MGC43342; R-spondin family; member 2; R-spondin 2 homolog (Xenopus laevis)
other names :
R-spondin-2 isoform 2; R-spondin-2; R-spondin-2; R-spondin 2 homolog; roof plate-specific spondin-2; R-spondin 2; Roof plate-specific spondin-2; hRspo2
products gene name :
RSPO2
other gene names :
RSPO2; RSPO2; CRISTIN2; UNQ9384/PRO34209; hRspo2
uniprot entry name :
RSPO2_HUMAN
reactivity :
Human
sequence length :
179
specificity :
This assay has high sensitivity and excellent specificity for detection of human RSPO2. No significant cross-reactivity or interference between human RSPO2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0. 156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human RSPO2 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RSPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RSPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RSPO2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RSPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
544583460
ncbi acc num :
NP_001269792.1
ncbi gb acc num :
NM_001282863.1
uniprot acc num :
Q6UXX9
ncbi mol weight :
28,315 Da
ncbi summary :
R-spondins (RSPOs), such as RSPO2, are secreted proteins that regulate beta-catenin (CTNNB1; MIM 116806) signaling (Kim et al., 2006 [PubMed 16357527]).[supplied by OMIM, Mar 2008]
uniprot summary :
R-spondin-2: Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway, probably by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Probably also acts as a ligand for frizzled and LRP receptors. Belongs to the R-spondin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Cell development/differentiation; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; cell surface; extracellular region. Molecular Function: heparin binding; G-protein-coupled receptor binding; receptor binding. Biological Process: embryonic forelimb morphogenesis; osteoblast differentiation; Wnt receptor signaling pathway; embryonic hindlimb morphogenesis; bone mineralization
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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