ELISA/assay

Mouse Follistatin-related protein 3, FSTL3 ELISA Kit

MBS911400

48-Strip-Wells

510 USD

ELISA Kit

Mouse Follistatin-related protein 3, FSTL3 ELISA Kit

follistatin-like 3 (secreted glycoprotein)

Mouse Follistatin-related protein 3 (FSTL3) ELISA kit; FLRG; FSRP; follistatin-like 3 glycoprotein; follistatin-related protein; follistatin-like 3 (secreted glycoprotein)

follistatin-related protein 3; Follistatin-related protein 3; follistatin-related protein 3; follistatin-like protein 3; follistatin-related gene protein; follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein

FSTL3

Fstl3; Fstl3; Flrg; E030038F23Rik; Flrg

FSTL3_MOUSE

Mouse

256

This assay has high sensitivity and excellent specificity for detection of mouse FSTL3. No significant cross-reactivity or interference between mouse FSTL3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4 ng/ml - 140 ng/ml. Sensitivity: The minimum detectable dose of mouse FSTL3 is typically less than 2 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse FSTL3 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FSTL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for FSTL3. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSTL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

13878203

NP_113557.1

NM_031380.2

Q9EQC7

27,271 Da

FSTL3: Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. A chromosomal aberration involving FSTL3 is found in a case of B-cell chronic lymphocytic leukemia. Translocation t(11;19)(q13;p13) with CCDN1. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Secreted; Oncoprotein; Secreted, signal peptide. Cellular Component: nucleoplasm; Golgi apparatus; extracellular space; extracellular region; nucleus; secretory granule. Molecular Function: fibronectin binding; activin binding. Biological Process: regulation of transcription from RNA polymerase II promoter; negative regulation of osteoclast differentiation; negative regulation of activin receptor signaling pathway; ossification; regulation of transcription, DNA-dependent; transcription, DNA-dependent; positive regulation of cell-cell adhesion; positive regulation of transcription from RNA polymerase II promoter; hemopoietic progenitor cell differentiation; negative regulation of BMP signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800