Human Carcinoembryonic antigen-related cell adhesion molecule 3, CEACAM3 ELISA Kit | MyBioSource MBS911348 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Carcinoembryonic antigen-related cell adhesion molecule 3, CEACAM3 ELISA Kit

catalog :

MBS911348

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911348

products type :

ELISA Kit

products full name :

Human Carcinoembryonic antigen-related cell adhesion molecule 3, CEACAM3 ELISA Kit

products short name :

carcinoembryonic antigen-related cell adhesion molecule 3

products name syn :

Human Carcinoembryonic antigen-related cell adhesion molecule 3 (CEACAM3) ELISA kit; CD66D; CEA; CGM1; MGC119875; W264; W282; CD66d antigen; carcinoembryonic antigen CGM1; carcinoembryonic antigen gene family member 1; nonspecific cross-reacting antigen; carcinoembryonic antigen-related cell adhesion molecule 3

other names :

carcinoembryonic antigen-related cell adhesion molecule 3 isoform 2; Carcinoembryonic antigen-related cell adhesion molecule 3; carcinoembryonic antigen-related cell adhesion molecule 3; CD66d antigen; carcinoembryonic antigen CGM1; nonspecific cross-reacting antigen; carcinoembryonic antigen gene family member 1; carcinoembryonic antigen-related cell adhesion molecule 3; Carcinoembryonic antigen CGM1; CD_antigen: CD66d

products gene name :

CEACAM3

other gene names :

CEACAM3; CEACAM3; CEA; CGM1; W264; W282; CD66D; CD66D; CGM1

uniprot entry name :

CEAM3_HUMAN

reactivity :

Human

sequence length :

212

specificity :

This assay has high sensitivity and excellent specificity for detection of human CEACAM3. No significant cross-reactivity or interference between human CEACAM3 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human CEACAM3 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CEACAM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CEACAM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CEACAM3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CEACAM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

461496471

ncbi acc num :

NP_001264092.1

ncbi gb acc num :

NM_001277163.2

uniprot acc num :

P40198

ncbi mol weight :

27,091 Da

ncbi summary :

This gene encodes a member of the family of carcinoembryonic antigen-related cell adhesion molecules (CEACAMs), which are used by several bacterial pathogens to bind and invade host cells. The encoded transmembrane protein directs phagocytosis of several bacterial species that is dependent on the small GTPase Rac. It is thought to serve an important role in controlling human-specific pathogens by the innate immune system. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2013]

uniprot summary :

CEACAM3: Major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neissiria, Moxarella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis. Interacts with S100A9/calprotectin. This interaction is calcium-dependent, but independent of CEACAM3 phosphorylation. CGM1a, the predominant CGM1 transcript, is granulocyte-specific. Not detected out of the granulocytic lineage, such as monocytes, lymphocytes, spleen, testis, colon, brain, liver, pancreas, thymus, ovary, placenta, skeletal muscle, prostate, small intestine, heart, lung and kidney. Belongs to the immunoglobulin superfamily. CEA family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cell adhesion; Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.2. Cellular Component: integral to membrane

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage