catalog number :
MBS911215
products type :
ELISA Kit
products full name :
Mouse proenkephalin A, PENK ELISA Kit
products short name :
proenkephalin A
products name syn :
Mouse proenkephalin A (PENK) ELISA kit; enkephalin A; preproenkephalin; proenkephalin A
other names :
proenkephalin-A; Proenkephalin-A; proenkephalin-A; preproenkephalin 1; spermatogenic-specific proenkephalin; preproenkephalin; Opioid growth factor; OGF
products gene name :
PENK
other gene names :
Penk; Penk; ENK; PPA; Penk1; AI326464; Penk1; OGF
uniprot entry name :
PENK_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse PENK. No significant cross-reactivity or interference between Mouse PENK and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml-800 pg/ml. Sensitivity: 3.12 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PENK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PENK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PENK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PENK bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001002927.1
ncbi gb acc num :
NM_001002927.2
ncbi mol weight :
31,004 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (575426); G Alpha (i) Signalling Events Pathway (575454); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); Peptide Ligand-binding Receptors Pathway (575427); Signal Transduction Pathway (575318); Signaling By GPCR Pathway (575424)
uniprot summary :
PENK: Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum. Belongs to the opioid neuropeptide precursor family. Protein type: Secreted, signal peptide; Secreted; Hormone
size4 :
10x96-Strip-Wells