Mouse Aminopeptidase N, ANPEP ELISA Kit | MyBioSource MBS911187 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Aminopeptidase N, ANPEP ELISA Kit

catalog :

MBS911187

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS911187

products type :

ELISA Kit

products full name :

Mouse Aminopeptidase N, ANPEP ELISA Kit

products short name :

alanyl (membrane) aminopeptidase

products name syn :

Mouse Aminopeptidase N (ANPEP) ELISA kit; APN; CD13; GP150; LAP1; P150; PEPN; aminopeptidase M; aminopeptidase N; membrane alanine aminopeptidase; microsomal aminopeptidase; myeloid plasma membrane glycoprotein CD13; alanyl (membrane) aminopeptidase

other names :

aminopeptidase N; Aminopeptidase N; aminopeptidase N; aminopeptidase M; alanyl aminopeptidase; aminopeptidase N/CD13; membrane protein p161; microsomal aminopeptidase; alanyl (membrane) aminopeptidase; Alanyl aminopeptidase; Aminopeptidase M; AP-M; Membrane protein p161; Microsomal aminopeptidase; CD_antigen: CD13

products gene name :

ANPEP

other gene names :

Anpep; Anpep; Apn; AP-M; AP-N; Cd13; P150; Lap-1; Lap1; AP-N; mAPN; AP-M

uniprot entry name :

AMPN_MOUSE

reactivity :

Mouse

sequence length :

966

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse ANPEP. No significant cross-reactivity or interference between mouse ANPEP and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 39 mU/ml -2500 mU/ml. Sensitivity: The minimum detectable dose of mouse ANPEP is typically less than 9.7 mU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANPEP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

225637487

ncbi acc num :

NP_032512.2

ncbi gb acc num :

NM_008486.2

uniprot acc num :

P97449

ncbi mol weight :

109,651 Da

ncbi pathways :

Glutathione Metabolism Pathway (83172); Glutathione Metabolism Pathway (198381); Glutathione Metabolism Pathway (343); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Renin-angiotensin System Pathway (83272); Renin-angiotensin System Pathway (486); Gamma-glutamyl Cycle Pathway (142875); Glutathione-mediated Detoxification Pathway (143044)

uniprot summary :

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family. Protein type: Membrane protein, integral; Protease; Other Amino Acids Metabolism - glutathione; EC 3.4.11.2; Receptor, misc. Cellular Component: extracellular space; membrane; lysosomal membrane; integral to membrane; ER-Golgi intermediate compartment; vesicle membrane; external side of plasma membrane. Molecular Function: peptidase activity; metallopeptidase activity; hydrolase activity; zinc ion binding; metal ion binding; aminopeptidase activity; peptide binding. Biological Process: multicellular organismal development; aromatic compound metabolic process; angiogenesis; cell differentiation; proteolysis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage