Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit | MyBioSource MBS910999 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

catalog :

MBS910999

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS910999

products type :

ELISA Kit

products full name :

Mouse Indoleamine 2, 3-dioxygenase 1, IDO1 ELISA Kit

products short name :

indoleamine 2, 3-dioxygenase 1

products name syn :

Mouse Indoleamine 2; 3-dioxygenase 1 (IDO1) ELISA kit; CD107B; IDO; INDO; indole 2; 3-dioxygenase; indoleamine-pyrrole 2; 3 dioxygenase; indoleamine 2; 3-dioxygenase 1

other names :

indoleamine 2,3-dioxygenase 1 isoform 1; Indoleamine 2,3-dioxygenase 1; indoleamine 2,3-dioxygenase 1; IDO-1; indole 2,3-dioxygenase; indoleamine-pyrrole 2,3 dioxygenase; indoleamine-pyrrole 2,3-dioxygenase; indoleamine 2,3-dioxygenase 1; Indoleamine-pyrrole 2,3-dioxygenase

products gene name :

IDO1

other gene names :

Ido1; Ido1; Ido; Indo; Ido; Indo; IDO-1

uniprot entry name :

I23O1_MOUSE

reactivity :

Mouse

sequence length :

407

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse IDO1. No significant cross-reactivity or interference between mouse IDO1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of mouse IDO1 is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IDO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IDO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IDO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IDO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6680347

ncbi acc num :

NP_032350.1

ncbi gb acc num :

NM_008324.2

uniprot acc num :

P28776

ncbi mol weight :

45,641 Da

ncbi pathways :

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Metabolic Pathways (132962); Metabolism Pathway (971233); Metabolism Of Amino Acids And Derivatives Pathway (970519); NAD Biosynthesis II (from Tryptophan) Pathway (142866); Tryptophan Catabolism Pathway (970873); Tryptophan Metabolism Pathway (83163); Tryptophan Metabolism Pathway (198332); Tryptophan Metabolism Pathway (332)

uniprot summary :

INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family. Protein type: Cell cycle regulation; Amino Acid Metabolism - tryptophan; EC 1.13.11.52; Oxidoreductase. Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytoplasm; cytosol. Molecular Function: amino acid binding; protein binding; dioxygenase activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; oxidoreductase activity; oxygen binding; tryptophan 2,3-dioxygenase activity. Biological Process: positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; tryptophan catabolic process to kynurenine; positive regulation of interleukin-12 production; positive regulation of apoptosis; negative regulation of activated T cell proliferation; response to lipopolysaccharide; defense response; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; inflammatory response; multicellular organismal response to stress; tryptophan catabolic process; positive regulation of T cell tolerance induction

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage