Human Galactose-1-phosphate uridylyltransferase, GALT ELISA Kit | MyBioSource MBS910947 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Galactose-1-phosphate uridylyltransferase, GALT ELISA Kit

catalog :

MBS910947

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS910947

products type :

ELISA Kit

products full name :

Human Galactose-1-phosphate uridylyltransferase, GALT ELISA Kit

products short name :

galactose-1-phosphate uridylyltransferase

products name syn :

Human Galactose-1-phosphate uridylyltransferase (GALT) ELISA kit; hCG_2040046; galactose-1-phosphate uridyl transferase; galactose-1-phosphate uridylyltransferase

other names :

galactose-1-phosphate uridylyltransferase isoform 1; Galactose-1-phosphate uridylyltransferase; galactose-1-phosphate uridylyltransferase; gal-1-P uridylyltransferase; galactose-1-phosphate uridyl transferase; UDP-glucose--hexose-1-phosphate uridylyltransferase; galactose-1-phosphate uridylyltransferase; UDP-glucose--hexose-1-phosphate uridylyltransferase

products gene name :

GALT

other gene names :

GALT; GALT; Gal-1-P uridylyltransferase

uniprot entry name :

GALT_HUMAN

reactivity :

Human

sequence length :

379

specificity :

This assay has high sensitivity and excellent specificity for detection of human GALT. No significant cross-reactivity or interference between human GALT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human GALT is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GALT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GALT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GALT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GALT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

22165416

ncbi acc num :

NP_000146.2

ncbi gb acc num :

NM_000155.3

uniprot acc num :

P07902

ncbi mol weight :

43,363 Da

ncbi pathways :

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Disease Pathway (530764); Galactose Catabolism Pathway (106219); Galactose Degradation, Leloir Pathway, Galactose = Alpha-D-glucose-1P (961175); Galactose Degradation, Leloir Pathway, Galactose = Alpha-D-glucose-1P (966887); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956)

ncbi summary :

Galactose-1-phosphate uridyl transferase (GALT) catalyzes the second step of the Leloir pathway of galactose metabolism, namely the conversion of UDP-glucose + galactose-1-phosphate to glucose-1-phosphate + UDP-galactose. The absence of this enzyme results in classic galactosemia in humans and can be fatal in the newborn period if lactose is not removed from the diet. The pathophysiology of galactosemia has not been clearly defined. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

uniprot summary :

GALT: Defects in GALT are the cause of galactosemia (GALCT). Galactosemia is an inherited disorder of galactose metabolism that causes jaundice, cataracts, and mental retardation. Belongs to the galactose-1-phosphate uridylyltransferase type 1 family. Protein type: Motility/polarity/chemotaxis; Carbohydrate Metabolism - galactose; EC 2.7.7.12; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase. Chromosomal Location of Human Ortholog: 9p13. Cellular Component: Golgi apparatus; cytosol. Molecular Function: zinc ion binding; UDP-glucose:hexose-1-phosphate uridylyltransferase activity. Biological Process: galactose catabolic process; UDP-glucose catabolic process; carbohydrate metabolic process; galactose metabolic process; pathogenesis. Disease: Galactosemia

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage