Human Allograft inflammatory factor 1, AIF1 ELISA Kit | MyBioSource MBS910912 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Allograft inflammatory factor 1, AIF1 ELISA Kit

catalog :

MBS910912

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS910912

products type :

ELISA Kit

products full name :

Human Allograft inflammatory factor 1, AIF1 ELISA Kit

products short name :

[allograft inflammatory factor 1]

products name syn :

[Human Allograft inflammatory factor 1 (AIF1) ELISA kit; DADB-70P7.8; AIF-1; IBA1; IRT-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule; allograft inflammatory factor 1]

other names :

[allograft inflammatory factor 1 isoform 3; Allograft inflammatory factor 1; allograft inflammatory factor 1; protein G1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; allograft inflammatory factor-1 splice variant Hara-1; allograft inflammatory factor 1; Ionized calcium-binding adapter molecule 1; Protein G1]

products gene name :

[AIF1]

other gene names :

[AIF1; AIF1; IBA1; IRT1; AIF-1; IRT-1; G1; IBA1; AIF-1]

uniprot entry name :

AIF1_HUMAN

reactivity :

Human

sequence length :

147

specificity :

This assay has high sensitivity and excellent specificity for detection of human AIF1. No significant cross-reactivity or interference between human AIF1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: Typically less than 1.9 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AIF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

14574568

ncbi acc num :

NP_001614.3

ncbi gb acc num :

NM_001623.3

uniprot acc num :

P55008

ncbi mol weight :

16,703 Da

ncbi pathways :

Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

uniprot summary :

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: lamellipodium; perinuclear region of cytoplasm; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup. Molecular Function: actin filament binding; calcium ion binding. Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; positive regulation of protein amino acid phosphorylation; response to axon injury; response to electrical stimulus; phagocytosis, engulfment; inflammatory response; negative regulation of apoptosis

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145