ELISA/assay

Mouse neuregulin-4, NRG4 ELISA Kit

MBS910811

48-Strip-Wells

510 USD

ELISA Kit

Mouse neuregulin-4, NRG4 ELISA Kit

neuregulin-4 (NRG4)

Mouse neuregulin-4 (NRG4) ELISA kit; DKFZp779N0541; DKFZp779N1944; HRG4; heregulin 4; neuregulin-4 (NRG4)

pro-neuregulin-4, membrane-bound isoform; Pro-neuregulin-4, membrane-bound isoform; pro-neuregulin-4, membrane-bound isoform; pro-NRG4; neuregulin 4

NRG4

Nrg4; Nrg4; AI552600; Pro-NRG4; NRG-4

NRG4_MOUSE

Mouse

115

This assay has high sensitivity and excellent specificity for detection of mouse NRG4. No significant cross-reactivity or interference between mouse NRG4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRG4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRG4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRG4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRG4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

14030777

NP_114391.1

NM_032002.2

Q9WTX4

12,744 Da

Adaptive Immune System Pathway (971210); Constitutive PI3K/AKT Signaling In Cancer Pathway (1000676); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214); Disease Pathway (970606); Downstream Signal Transduction Pathway (971469); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1001193); Downstream Signaling Of Activated FGFR Pathway (971322); ErbB Signaling Pathway (83246); ErbB Signaling Pathway (198382)

NRG4: Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors. Belongs to the neuregulin family. Protein type: Membrane protein, integral. Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane; extracellular region; intracellular. Molecular Function: growth factor activity; receptor binding. Biological Process: organ development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800