product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse neuromedin B, NMB ELISA Kit
catalog :
MBS910678
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS910678
products type :
ELISA Kit
products full name :
Mouse neuromedin B, NMB ELISA Kit
products short name :
neuromedin B
products name syn :
Mouse neuromedin B (NMB) ELISA kit; MGC17211; MGC2277; MGC3936; ; neuromedin B
other names :
neuromedin-B isoform 2; Neuromedin-B; neuromedin-B; neuromedin B
products gene name :
NMB
other gene names :
Nmb; Nmb; 3110023K12Rik
uniprot entry name :
NMB_MOUSE
reactivity :
Mouse
sequence length :
121
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse NMB. No significant cross-reactivity or interference between mouse NMB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of mouse NMB is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Princple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NMB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NMB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NMB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NMB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
13386018
ncbi acc num :
NP_080799.1
ncbi gb acc num :
NM_026523.4
uniprot acc num :
Q9CR53
ncbi mol weight :
13,571 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (575426); G Alpha (q) Signalling Events Pathway (575456); GPCR Downstream Signaling Pathway (575452); GPCR Ligand Binding Pathway (575425); Peptide Ligand-binding Receptors Pathway (575427); Signal Transduction Pathway (575318); Signaling By GPCR Pathway (575424)
uniprot summary :
NMB: Stimulates smooth muscle contraction in a manner similar to that of bombesin. Belongs to the bombesin/neuromedin-B/ranatensin family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: neuron projection; extracellular region; cytosol. Molecular Function: neuromedin B receptor binding. Biological Process: elevation of cytosolic calcium ion concentration; neuropeptide signaling pathway; negative regulation of hormone secretion; positive regulation of cell proliferation; positive regulation of hormone secretion; arachidonic acid secretion; glucose homeostasis
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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