This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Leukocyte surface antigen CD47, CD47 ELISA Kit
catalog :
MBS910626
quantity :
96 Strip Wells
price :
760 USD
product information
catalog number :
MBS910626
products type :
ELISA Kit
products full name :
Rat Leukocyte surface antigen CD47, CD47 ELISA Kit
products short name :
CD47 molecule
products name syn :
Rat Leukocyte surface antigen CD47 (CD47) ELISA kit; IAP; MER6; OA3; CD47 antigen; CD47 antigen (Rh-related antigen; integrin-associated signal transducer) ; CD47 glycoprotein; Rh-related antigen; antigen identified by monoclonal antibody 1D8; antigenic sur; CD47 molecule
other names :
leukocyte surface antigen CD47; Leukocyte surface antigen CD47; leukocyte surface antigen CD47; IAP; integrin-associated protein; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); Cd47 molecule; Integrin-associated protein; IAP; CD_antigen: CD47
products gene name :
CD47
other gene names :
Cd47; Cd47; Itgp; IAP
uniprot entry name :
CD47_RAT
reactivity :
Rat
sequence length :
303
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat CD47. No significant cross-reactivity or interference between Rat CD47 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 12.5 pg/ml-800 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.12 pg/ml
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD47 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD47 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD47 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD47 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
9506469
ncbi acc num :
NP_062068.1
ncbi gb acc num :
NM_019195.2
uniprot acc num :
P97829
ncbi mol weight :
32,995 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway 1010919!!Cell-Cell Communication Pathway 1010357!!ECM-receptor Interaction Pathway 83460!!ECM-receptor Interaction Pathway 479!!Extracellular Matrix Organization Pathway 1009923!!Hemostasis Pathway 1010891!!Integrin Cell Surface Interactions Pathway 1009939!!Signal Regulatory Protein (SIRP) Family Interactions Pathway 1010367!!Spinal Cord Injury Pathway 739006
ncbi summary :
protein that may be involved in regulating renal cell growth; overexpression is associated with Fe-NTA-induced renal damage and tumor progression and metastasis [RGD, Feb 2006]
uniprot summary :
Function: Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection Plays an important role in memory formation and synaptic plasticity in the hippocampus.1 PublicationManual assertion based on experiment in:Ref.2
size :
96 Strip Wells
price :
760 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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