ELISA/assay

Mouse Cystatin-F, CST7 ELISA Kit

MBS910625

48-Strip-Wells

510 USD

ELISA Kit

Mouse Cystatin-F, CST7 ELISA Kit

cystatin F (leukocystatin)

Mouse Cystatin-F (CST7) ELISA kit; CMAP; cystatin F; cystatin-7; cystatin-F; cystatin-like metastasis-associated protein; cystatin F (leukocystatin)

cystatin-F; Cystatin-F; cystatin-F; cystatin 7; cystatin-7; cystatin-like metastasis-associated protein; cystatin F (leukocystatin); Cystatin-7; Cystatin-like metastasis-associated protein; CMAP; Leukocystatin

CST7

Cst7; Cst7; Cmap; CMAP

CYTF_MOUSE

Mouse

144

This assay has high sensitivity and excellent specificity for detection of mouse CST7. No significant cross-reactivity or interference between mouse CST7 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of mouse CST7 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CST7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CST7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CST7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CST7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

262263319

NP_034107.2

NM_009977.3

O89098

16,380 Da

CST7: Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system. Belongs to the cystatin family. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: protease inhibitor activity; cysteine protease inhibitor activity. Biological Process: negative regulation of peptidase activity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800