catalog number :
MBS910546
products type :
ELISA Kit
products full name :
Rat WAP four-disulfide core domain protein 2, WFDC2 ELISA Kit
products short name :
WAP four-disulfide core domain 2
products name syn :
Rat WAP four-disulfide core domain protein 2 (WFDC2) ELISA kit; EDDM4; HE4; MGC57529; WAP5; dJ461P17.6; WAP domain containing protein HE4-V4; epididymal protein 4; epididymal secretory protein E4; epididymis-specific; whey-acidic protein type; four-disulfide core; m; WAP four-disulfide core domain 2
other names :
WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; WAP four-disulfide core domain protein 2; epididymal protein BE-20; major epididymis-specific protein E4; Epididymal protein BE-20; Major epididymis-specific protein E4
products gene name :
WFDC2
other gene names :
WFDC2; WFDC2; BE-20
uniprot entry name :
WFDC2_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat WFDC2. No significant cross-reactivity or interference between Rat WFDC2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 12.5 pg/ml-800 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.125 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for WFDC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any WFDC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFDC2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFDC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001075823.1
ncbi gb acc num :
NM_001082354.1
ncbi mol weight :
12,803 Da
uniprot summary :
Function: Broad range protease inhibitor