Human Polycomb complex protein BMI-1, BMI1 ELISA Kit | MyBioSource MBS910433 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Polycomb complex protein BMI-1, BMI1 ELISA Kit

catalog :

MBS910433

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS910433

products type :

ELISA Kit

products full name :

Human Polycomb complex protein BMI-1, BMI1 ELISA Kit

products short name :

[BMI1 polycomb ring finger oncogene]

products name syn :

[Human Polycomb complex protein BMI-1 (BMI1) ELISA kit; RP11-573G6.1; MGC12685; PCGF4; RNF51; B lymphoma Mo-MLV insertion region 1 homolog; flvi-2/bmi-1; murine leukemia viral (bmi-1) oncogene homolog; oncogene BMI-1; polycomb group protein Bmi1; polycomb gro; BMI1 polycomb ring finger oncogene]

other names :

[COMMD3-BMI1 read-through protein; Polycomb complex protein BMI-1; COMMD3-BMI1 read-through protein; COMMD3-BMI1 readthrough; Polycomb group RING finger protein 4; RING finger protein 51]

products gene name :

[BMI1]

other gene names :

[COMMD3-BMI1; BMI1; PCGF4; RNF51]

uniprot entry name :

BMI1_HUMAN

reactivity :

Human

sequence length :

469

specificity :

This assay has high sensitivity and excellent specificity for detection of human BLC-1. No significant cross-reactivity or interference between human BLC-1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BLC-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BLC-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BLC-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BLC-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

323276616

ncbi acc num :

NP_001190991.1

ncbi gb acc num :

NM_001204062.1

uniprot acc num :

P35226

ncbi mol weight :

36,949 Da

ncbi pathways :

MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928); Senescence And Autophagy Pathway (198780); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Validated Targets Of C-MYC Transcriptional Activation Pathway (169351)

ncbi summary :

This locus represents naturally occurring read-through transcription between the neighboring COMM domain-containing protein 3 and polycomb complex protein BMI-1 genes on chromosome 10. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Feb 2011]

uniprot summary :

BMI1: Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A Lys-119 , rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. Component of a PRC1-like complex. Interacts with RING1 and RING2. Interacts vwith CBX7 and CBX8. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1. Protein type: Motility/polarity/chemotaxis; Ligase; Ubiquitin ligase; EC 6.3.2.-; Oncoprotein; Ubiquitin conjugating system. Chromosomal Location of Human Ortholog: 10p11.23. Cellular Component: nucleoplasm; nuclear body; heterochromatin; cytoplasm; PcG protein complex; nucleus; ubiquitin ligase complex. Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; ubiquitin-protein ligase activity; chromatin binding. Biological Process: transcription, DNA-dependent; in utero embryonic development; positive regulation of immature T cell proliferation in the thymus; negative regulation of transcription from RNA polymerase II promoter; humoral immune response; embryonic skeletal morphogenesis; segment specification; rostrocaudal neural tube patterning; positive regulation of fibroblast proliferation; regulation of gene expression; positive regulation of ubiquitin-protein ligase activity; histone ubiquitination; positive regulation of B cell proliferation; DNA methylation; somatic stem cell division; hemopoiesis; histone acetylation; brain development

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145