product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Neutrophil defensin 3, DEFA3 ELISA Kit
catalog :
MBS910392
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS910392
products type :
ELISA Kit
products full name :
Mouse Neutrophil defensin 3, DEFA3 ELISA Kit
products short name :
defensin, alpha 3, neutrophil-specific
products name syn :
Mouse Neutrophil defensin 3 (DEFA3) ELISA kit; DEF3; HNP-3; HNP3; HP-3; defensin 3; neutrophil-specific; defensin; alpha 3; neutrophil peptide 3; defensin; alpha 3; neutrophil-specific
other names :
alpha-defensin 17 preproprotein; Alpha-defensin 3; alpha-defensin 17; cryptdin 17; crypt defensin 17; defensin-related cryptdin-17; defensin, alpha, 17; Defensin-related cryptdin-3
products gene name :
DEFA3
other gene names :
Defa17; Defa3; Cryp17; Defcr17; AU014719; Defcr3
uniprot entry name :
DEFA3_MOUSE
reactivity :
Mouse
sequence length :
93
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse DEFA3. No significant cross-reactivity or interference between mouse DEFA3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of mouse DEFA3 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
268607652
ncbi acc num :
NP_001161262.1
ncbi gb acc num :
NM_001167790.1
uniprot acc num :
P28310
ncbi mol weight :
10,528 Da
ncbi pathways :
Alpha-defensins Pathway (1001261); Defensins Pathway (1001260); Immune System Pathway (971216); Innate Immune System Pathway (971212)
uniprot summary :
defensin, alpha 27: . Cellular Component: extracellular space. Biological Process: response to drug; defense response to Gram-positive bacterium; defense response to bacterium; defense response to Gram-negative bacterium
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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