ELISA/assay

Human Phosphoglucomutase-1, PGM1 ELISA Kit

MBS910386

96 Strip Wells

760 USD

ELISA Kit

Human Phosphoglucomutase-1, PGM1 ELISA Kit

phosphoglucomutase 1

Human Phosphoglucomutase-1 (PGM1) ELISA kit; GSD14; ; phosphoglucomutase 1

phosphoglucomutase-1 isoform 2; Phosphoglucomutase-1; phosphoglucomutase-1; PGM 1; glucose phosphomutase 1; phosphoglucomutase 1; Glucose phosphomutase 1

PGM1

PGM1; PGM1; CDG1T; GSD14; PGM 1

PGM1_HUMAN

Human

580

This assay has high sensitivity and excellent specificity for detection of Human PGM1. No significant cross-reactivity or interference between Human PGM1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 23.5 pg/ml-1500 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 5.8 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: glyconeogenesis and glycometabolism. Protein Biological Process 3: Carbohydrate metabolism

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PGM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PGM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PGM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PGM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

290463102

NP_001166289.1

NM_001172818.1

P36871

61,449 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway 82979!!Amino Sugar And Nucleotide Sugar Metabolism Pathway 350!!Disease Pathway 530764!!GDP-glucose Biosynthesis Pathway 142190!!Galactose Catabolism Pathway 106219!!Galactose Metabolism Pathway 82931!!Galactose Metabolism Pathway 292!!Glucose Metabolism Pathway 106199!!Glucuronidation Pathway 198821!!Glycogen Metabolism Pathway 198856

The protein encoded by this gene is an isozyme of phosphoglucomutase (PGM) and belongs to the phosphohexose mutase family. There are several PGM isozymes, which are encoded by different genes and catalyze the transfer of phosphate between the 1 and 6 positions of glucose. In most cell types, this PGM isozyme is predominant, representing about 90% of total PGM activity. In red cells, PGM2 is a major isozyme. This gene is highly polymorphic. Mutations in this gene cause glycogen storage disease type 14. Alternativley spliced transcript variants encoding different isoforms have been identified in this gene.[provided by RefSeq, Mar 2010]

Function: This enzyme participates in both the breakdown and synthesis of glucose. Catalytic activity: Alpha-D-glucose 1-phosphate = alpha-D-glucose 6-phosphate. Cofactor: Binds 1 magnesium ion per subunit.

96 Strip Wells

760 USD