Mouse Phospholipase A2, membrane associated, PLA2G2A ELISA Kit | MyBioSource MBS910298 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Phospholipase A2, membrane associated, PLA2G2A ELISA Kit

catalog :

MBS910298

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS910298

products type :

ELISA Kit

products full name :

Mouse Phospholipase A2, membrane associated, PLA2G2A ELISA Kit

products short name :

phospholipase A2, group IIA (platelets, synovial fluid)

products name syn :

Mouse Phospholipase A2; membrane associated (PLA2G2A) ELISA kit; MOM1; PLA2; PLA2B; PLA2L; PLA2S; PLAS1; sPLA2; phosphatidylcholine 2-acylhydrolase; phospholipase A2; group IIA; phospholipase A2; group IIA (platelets; synovial fluid)

other names :

phospholipase A2, membrane associated; Phospholipase A2, membrane associated; phospholipase A2, membrane associated; GIIC sPLA2; enhancing factor; modifier of Min1; group IIA phospholipase A2; phospholipase A2 group IIa; secretory group II phospholipase A2; phosphatidylcholine 2-acylhydrolase 2A; non-pancreatic secreted type II phospholipase A2; phospholipase A2, group IIA (platelets, synovial fluid); Enhancing factor; EF; GIIC sPLA2; Group IIA phospholipase A2; Phosphatidylcholine 2-acylhydrolase 2A

products gene name :

PLA2G2A

other gene names :

Pla2g2a; Pla2g2a; EF; Mom1; Pla2; sPLA2; sPla2-IIA; EF

uniprot entry name :

PA2GA_MOUSE

reactivity :

Mouse

sequence length :

146

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G2A. No significant cross-reactivity or interference between Mouse PLA2G2A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.312 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Growth Factor. Protein Biological Process 3: Growth regulation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2G2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2G2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G2A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

132626633

ncbi acc num :

NP_001076000.1

ncbi gb acc num :

NM_001082531.1

uniprot acc num :

P31482

ncbi mol weight :

16,145 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198357); Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Glycerophospholipid Metabolism Pathway (83188); Glycerophospholipid Metabolism Pathway (364); Linoleic Acid Metabolism Pathway (83191)

ncbi summary :

Proteins belonging to the phospholipase A2 (PLA2) family hydrolyze phospholipids into sn2 fatty acids and lysophospholipids. They function in a variety of cellular processes, including the digestion of phospholipids and the production of molecules that induce inflammatory responses. This gene encodes a member of the group II class of secretory PLA2s. The secreted enzyme binds to heparin on the cell surface. Mutations in this gene increase the occurrence of intestinal polyps caused by a dominant mutation in the adenomatosis polyposis coli gene. A frameshift inactivates this gene product in some mouse strains including the strain of the reference genome, C57BL/6J, whereas a functional protein is produced in other strains. [provided by RefSeq, Jul 2008]

uniprot summary :

PLA2G2A: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Belongs to the phospholipase A2 family. Protein type: Secreted, signal peptide; Lipid Metabolism - alpha-linolenic acid; Vesicle; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Lipid Metabolism - linoleic acid; Mitochondrial; Phospholipase; Lipid Metabolism - ether lipid; Lipid Metabolism - glycerophospholipid; Endoplasmic reticulum; Secreted. Cellular Component: mitochondrion; membrane. Molecular Function: phospholipase A2 activity; hydrolase activity; metal ion binding; calcium ion binding. Biological Process: negative regulation of cell proliferation; somatic stem cell maintenance; regulation of growth; phospholipid metabolic process; lipid metabolic process; regulation of epithelial cell proliferation; lipid catabolic process; regulation of cell proliferation; negative regulation of epithelial cell proliferation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage