ELISA/assay

Human Zinc finger E-box-binding homeobox 1, ZEB1 ELISA Kit

MBS910271

96 Strip Wells

760 USD

ELISA Kit

Human Zinc finger E-box-binding homeobox 1, ZEB1 ELISA Kit

zinc finger E-box binding homeobox 1

Human Zinc finger E-box-binding homeobox 1 (ZEB1) ELISA kit; RP11-472N13.4; AREB6; BZP; DELTA-EF1; MGC133261; NIL-2-A; NIL-2A; NIL2A; TCF8; ZEB; ZFHEP; ZFHX1A; transcription factor 8 (represses interleukin 2 expression) ; zinc finger homeodomain enhancer-bindin; zinc finger E-box binding homeobox 1

zinc finger E-box-binding homeobox 1 isoform a; Zinc finger E-box-binding homeobox 1; zinc finger E-box-binding homeobox 1; negative regulator of IL2; delta-crystallin enhancer binding factor 1; posterior polymorphous corneal dystrophy 3; zinc finger homeodomain enhancer-binding protein; transcription factor 8 (represses interleukin 2 expression); zinc finger E-box binding homeobox 1; NIL-2-A zinc finger protein; Negative regulator of IL2; Transcription factor 8; TCF-8

ZEB1

ZEB1; ZEB1; BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1; AREB6; TCF8; TCF-8

ZEB1_HUMAN

Human

1108

This assay has high sensitivity and excellent specificity for detection of human ZEB1. No significant cross-reactivity or interference between human ZEB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human zinc finger E-box-binding homeobox 1 (ZEB1) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 25 pg/ml -1600 pg/ml.

Sensitivity: The minimum detectable dose of human ZEB1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ZEB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZEB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZEB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZEB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

189409130

NP_001121600.1

NM_001128128.2

P37275

124,074 Da

Hypertrophy Model Pathway 198828!!MicroRNAs In Cancer Pathway 852705!!MicroRNAs In Cancer Pathway 852928!!TGF-beta Receptor Signaling Pathway 198774!!Transcriptional Misregulation In Cancer Pathway 523016!!Transcriptional Misregulation In Cancer Pathway 522987

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Mar 2010]

Function: Acts as a transcriptional repressor. Inhibits interleukin-2 (IL-2) gene expression. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. Represses RCOR1 transcription activation during neurogenesis. Represses transcription by binding to the E box (5'-CANNTG-3'). Promotes tumorigenicity by repressing stemness-inhibiting microRNAs.3 PublicationsManual assertion based on experiment in:Ref.13

96 Strip Wells

760 USD