ELISA/assay

Human Nuclear receptor ROR-alpha, RORA ELISA Kit

MBS910208

48-Strip-Wells

510 USD

ELISA Kit

Human Nuclear receptor ROR-alpha, RORA ELISA Kit

RAR-related orphan receptor A

Human Nuclear receptor ROR-alpha (RORA) ELISA kit; DKFZp686M2414; MGC119326; MGC119329; NR1F1; ROR1; ROR2; ROR3; RZR-ALPHA; RZRA; RAR-related orphan receptor alpha; ROR-alpha; retinoic acid receptor-related orphan receptor alpha; retinoid-related orpha; RAR-related orphan receptor A

nuclear receptor ROR-alpha isoform c; Nuclear receptor ROR-alpha; nuclear receptor ROR-alpha; nuclear receptor RZR-alpha; transcription factor RZR-alpha; retinoid-related orphan receptor alpha; nuclear receptor subfamily 1 group F member 1; thyroid hormone nuclear receptor alpha variant 4; retinoic acid receptor-related orphan receptor alpha; RAR-related orphan receptor A; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha

RORA

RORA; RORA; ROR1; ROR2; ROR3; RZRA; NR1F1; RZR-ALPHA; NR1F1; RZRA

RORA_HUMAN

Human

548

This assay has high sensitivity and excellent specificity for detection of Human RORA. No significant cross-reactivity or interference between Human RORA and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.68 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for RORA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RORA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RORA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RORA bound in the initial step. The color development is stopped and the intensity of the color is measured.

4506577

NP_002934.1

NM_002943.3

P35398

58,975 Da

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); Circadian Rhythm Pathway (83084); Circadian Rhythm Pathway (495); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Inflammatory Bowel Disease (IBD) Pathway (842771); Inflammatory Bowel Disease (IBD) Pathway (842797)

The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

RORA iso1: Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5 -[AT][TA]A[AT][CGT]TAGGTCA-3 . Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. Monomer. Interacts (via the DNA-binding domain) with HIF1; the interaction enhances HIF1A transcription under hypoxia through increasing protein stability. By hypoxia and melatonin. Widely expressed in a number of tissues. Activated by CaMK4. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Nuclear receptor. Chromosomal Location of Human Ortholog: 15q22.2. Cellular Component: nucleoplasm; nucleus. Molecular Function: ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; beta-catenin binding; steroid hormone receptor activity; oxysterol binding; transcription factor activity; transcription factor binding. Biological Process: circadian rhythm; transcription initiation from RNA polymerase II promoter; granule cell precursor proliferation; intracellular receptor-mediated signaling pathway; regulation of macrophage activation; positive regulation of transcription, DNA-dependent; positive regulation of circadian rhythm; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of fat cell differentiation; muscle cell differentiation; regulation of transcription, DNA-dependent; xenobiotic metabolic process; negative regulation of inflammatory response; regulation of steroid metabolic process; cerebellar Purkinje cell differentiation; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; angiogenesis; circadian regulation of gene expression; nitric oxide biosynthetic process; cGMP metabolic process; regulation of smoothened signaling pathway

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800