catalog number :
MBS910099
products type :
ELISA Kit
products full name :
Bovine Apolipoprotein A-IV, APOA4 ELISA Kit
products short name :
apolipoprotein A-IV
products name syn :
Bovine Apolipoprotein A-IV (APOA4) ELISA kit; MGC142154; MGC142156; ; apolipoprotein A-IV
other names :
apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV<; Apolipoprotein A4
products gene name :
APOA4
other gene names :
APOA4; APOA4; Apo-AIV; ApoA-IV
uniprot entry name :
APOA4_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine APOA4. No significant cross-reactivity or interference between bovine APOA4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of bovine apolipoprotein A-IV (APOA4) concentrations in serum, plasma, tissue homogenates. Detection Range: 1.56 ug/ml -100 ug/ml.
other info2 :
Sensitivity: The minimum detectable dose of bovine APOA4 is typically less than 0.39 ug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA4 present is bound by the immobilized antibody. After removing any unbound substances, a Horseradish Peroxidase (HRP)-conjugated antibody specific for APOA4 is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001032557.1
ncbi gb acc num :
NM_001037480.1
ncbi mol weight :
43,018 Da
ncbi pathways :
Amyloids Pathway 1008671!!Chylomicron-mediated Lipid Transport Pathway 1009292!!Disease Pathway 942846!!Diseases Associated With Visual Transduction Pathway 1008713!!Fat Digestion And Absorption Pathway 194380!!Fat Digestion And Absorption Pathway 194324!!Lipid Digestion, Mobilization, And Transport Pathway 1009288!!Lipoprotein Metabolism Pathway 1009291!!Metabolism Pathway 942801!!Metabolism Of Lipids And Lipoproteins Pathway 942902
uniprot summary :
Function: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons