Human Protein argonaute-2, EIF2C2 ELISA Kit | MyBioSource MBS910054 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein argonaute-2, EIF2C2 ELISA Kit

catalog :

MBS910054

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS910054

products type :

ELISA Kit

products full name :

Human Protein argonaute-2, EIF2C2 ELISA Kit

products short name :

eukaryotic translation initiation factor 2C, 2

products name syn :

Human Protein argonaute-2 (EIF2C2) ELISA kit; AGO2; MGC3183; Q10; argonaute 2; eukaryotic translation initiation factor 2C; 2

other names :

protein argonaute-2 isoform 2; Protein argonaute-2; protein argonaute-2; PPD; hAgo2; eIF2C 2; eIF-2C 2; argonaute2; argonaute 2; protein slicer; PAZ Piwi domain protein; eukaryotic translation initiation factor 2C, 2; argonaute RISC catalytic component 2; Argonaute RISC catalytic component 2; Eukaryotic translation initiation factor 2C 2; eIF-2C 2; eIF2C 2; PAZ Piwi domain protein; PPD; Protein slicer

products gene name :

EIF2C2

other gene names :

AGO2; AGO2; Q10; EIF2C2; EIF2C2; Argonaute2; hAgo2; eIF-2C 2; eIF2C 2; PPD

uniprot entry name :

AGO2_HUMAN

reactivity :

Human

sequence length :

825

specificity :

This assay has high sensitivity and excellent specificity for detection of human EIF2C2. No significant cross-reactivity or interference between human EIF2C2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml-1600 pg/ml. Sensitivity: Typically less than 6.25 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EIF2C2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EIF2C2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EIF2C2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EIF2C2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

257467482

ncbi acc num :

NP_001158095.1

ncbi gb acc num :

NM_001164623.1

uniprot acc num :

Q9UKV8

ncbi mol weight :

97,208 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Ca2+ Pathway (941399); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); Fc Epsilon Receptor (FCERI) Signaling Pathway (833825); GAB1 Signalosome Pathway (106341)

ncbi summary :

This gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

uniprot summary :

AGO2: the endonuclease in RNA-induced silencing complexes (RISC) that cleaves siRNA/mRNA heteroduplexes bound to RISC. Ago2, along with Dicer and TRBP, are major components of RISC. Interacts with Dicer1 through its Piwi domain. Required for proper fibroblast growth factor signaling during gastrulation. Essential for embryonic development as well as RNA-mediated gene silencing (RNAi). Protein type: RNA processing; RNA-binding; EC 3.1.26.n2. Chromosomal Location of Human Ortholog: 8q24. Cellular Component: nucleoplasm; polysome; mRNA cap complex; membrane; cytoplasm; ribonucleoprotein complex; cytosol. Molecular Function: mRNA binding; endoribonuclease activity; protein binding; miRNA binding; siRNA binding; metal ion binding; translation initiation factor activity; RNA 7-methylguanosine cap binding. Biological Process: epidermal growth factor receptor signaling pathway; Notch signaling pathway; negative regulation of translational initiation; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; translation; transcription, DNA-dependent; RNA interference, siRNA loading onto RISC; nerve growth factor receptor signaling pathway; miRNA-mediated gene silencing, mRNA cleavage; miRNA-mediated gene silencing, miRNA loading onto RISC; miRNA-mediated gene silencing, negative regulation of translation; post-embryonic development; regulation of transcription, DNA-dependent; pre-microRNA processing; translational initiation; innate immune response; RNA-mediated gene silencing; gene expression; posttranscriptional gene silencing

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage