Human heparan sulfate proteoglycan 2, HSPG2 ELISA Kit | MyBioSource MBS910014 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human heparan sulfate proteoglycan 2, HSPG2 ELISA Kit

catalog :

MBS910014

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS910014

products type :

ELISA Kit

products full name :

Human heparan sulfate proteoglycan 2, HSPG2 ELISA Kit

products short name :

heparan sulfate proteoglycan 2

products name syn :

Human heparan sulfate proteoglycan 2 (HSPG2) ELISA kit; RP11-132G19.2; PLC; PRCAN; SJA; SJS; SJS1; Schwartz-Jampel syndrome 1 (chondrodystrophic myotonia) ; endorepellin (domain V region) ; heparan sulfate proteoglycan of basement membrane; perlecan proteogly; heparan sulfate proteoglycan 2

other names :

basement membrane-specific heparan sulfate proteoglycan core protein isoform a; Basement membrane-specific heparan sulfate proteoglycan core protein; basement membrane-specific heparan sulfate proteoglycan core protein; perlecan proteoglycan; endorepellin (domain V region); heparan sulfate proteoglycan 2; Perlecan; PLC

products gene name :

HSPG2

other gene names :

HSPG2; HSPG2; PLC; SJA; SJS; HSPG; SJS1; PRCAN; HSPG; PLC

uniprot entry name :

PGBM_HUMAN

reactivity :

Human

sequence length :

4392

specificity :

This assay has high sensitivity and excellent specificity for detection of Human HSPG2. No significant cross-reactivity or interference between Human HSPG2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 62.5 pg/ml-4000 pg/ml. Sensitivity: 15.6 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPG2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

629266065

ncbi acc num :

NP_001278789.1

ncbi gb acc num :

NM_001291860.1

uniprot acc num :

P98160

ncbi mol weight :

468,830 Da

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (645305); Amyloids Pathway (366238); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (645308); Chylomicron-mediated Lipid Transport Pathway (106157); Degradation Of The Extracellular Matrix Pathway (576263); Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes the perlecan protein, which consists of a core protein to which three long chains of glycosaminoglycans (heparan sulfate or chondroitin sulfate) are attached. The perlecan protein is a large multidomain proteoglycan that binds to and cross-links many extracellular matrix components and cell-surface molecules. It has been shown that this protein interacts with laminin, prolargin, collagen type IV, FGFBP1, FBLN2, FGF7 and transthyretin, etc., and it plays essential roles in multiple biological activities. Perlecan is a key component of the vascular extracellular matrix, where it helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. It can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation. It is a major component of basement membranes, where it is involved in the stabilization of other molecules as well as being involved with glomerular permeability to macromolecules and cell adhesion. Mutations in this gene cause Schwartz-Jampel syndrome type 1, Silverman-Handmaker type of dyssegmental dysplasia, and tardive dyskinesia. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014]

uniprot summary :

HSPG2: Integral component of basement membranes. Component of the glomerular basement membrane (GBM), responsible for the fixed negative electrostatic membrane charge, and which provides a barrier which is both size- and charge-selective. It serves as an attachment substrate for cells. Plays essential roles in vascularization. Critical for normal heart development and for regulating the vascular response to injury. Also required for avascular cartilage development. Defects in HSPG2 are the cause of Schwartz-Jampel syndrome (SJS1); a rare autosomal recessive disorder characterized by permanent myotonia (prolonged failure of muscle relaxation) and skeletal dysplasia, resulting in reduced stature, kyphoscoliosis, bowing of the diaphyses and irregular epiphyses. Defects in HSPG2 are the cause of dyssegmental dysplasia Silverman-Handmaker type (DDSH). The dyssegmental dysplasias are rare, autosomal recessive skeletal dysplasias with anisospondyly and micromelia. There are two recognized types: the severe, lethal DDSH and the milder Rolland-Desbuquois form. Individuals with DDSH also have a flat face, micrognathia, cleft palate and reduced joint mobility, and frequently have an encephalocoele. The endochondral growth plate is short, the calcospherites (which are spherical calcium-phosphorus crystals produced by hypertrophic chondrocytes) are unfused, and there is mucoid degeneration of the resting cartilage. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 1p36.1-p34. Cellular Component: extracellular matrix; extracellular space; lysosomal lumen; focal adhesion; Golgi lumen; extracellular region; plasma membrane; basal lamina. Molecular Function: protein C-terminus binding; protein binding; metal ion binding. Biological Process: cardiac muscle development; phototransduction, visible light; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; lipoprotein metabolic process; pathogenesis; embryonic skeletal morphogenesis; chondroitin sulfate metabolic process; glycosaminoglycan biosynthetic process; extracellular matrix disassembly; glycosaminoglycan catabolic process; protein localization; carbohydrate metabolic process; chondrocyte differentiation; angiogenesis; brain development; retinoid metabolic process; endochondral ossification. Disease: Schwartz-jampel Syndrome, Type 1; Dyssegmental Dysplasia, Silverman-handmaker Type

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage