Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4 ELISA Kit | MyBioSource MBS909843 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4 ELISA Kit

catalog :

MBS909843

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909843

products type :

ELISA Kit

products full name :

Mouse Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, GPX4 ELISA Kit

products short name :

glutathione peroxidase 4 (phospholipid hydroperoxidase)

products name syn :

Mouse Phospholipid hydroperoxide glutathione peroxidase; mitochondrial (GPX4) ELISA kit; MCSP; PHGPx; snGPx; snPHGPx; glutathione peroxidase 4; phospholipid hydroperoxidase; phospholipid hydroperoxide glutathione peroxidase; mitochondrial; sperm nucleus glutathione peroxidase; glutathione peroxidase 4 (phospholipid hydroperoxidase)

other names :

phospholipid hydroperoxide glutathione peroxidase, nuclear isoform 1; Phospholipid hydroperoxide glutathione peroxidase, nuclear; phospholipid hydroperoxide glutathione peroxidase, nuclear; sperm nuclei glutathione peroxidase; phospholipid hydroperoxide glutathione peroxidase, mitochondrial; glutathione peroxidase 4; Glutathione peroxidase 4; GPx-4; GSHPx-4

products gene name :

GPX4

other gene names :

Gpx4; Gpx4; GPx-4; PHGPx; snGPx; GSHPx-4; mtPHGPx; GPx-4; GSHPx-4

uniprot entry name :

GPX42_MOUSE

reactivity :

Mouse

sequence length :

253

specificity :

This assay has high sensitivity and excellent specificity for detection of Mouse GPX4. No significant cross-reactivity or interference between Mouse GPX4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPX4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPX4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPX4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPX4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

90903233

ncbi acc num :

NP_001032830.2

ncbi gb acc num :

NM_001037741.3

uniprot acc num :

Q91XR9

ncbi mol weight :

29,252 Da

ncbi pathways :

Folic Acid Network Pathway (198383); Glutathione Metabolism Pathway (83172); Glutathione Metabolism Pathway (198381); Glutathione Metabolism Pathway (343); One Carbon Metabolism And Related Pathways (198380); Selenium Metabolism/Selenoproteins Pathway (198289); Glutathione Redox Reactions I Pathway (142876); Selenium Pathway (198359)

ncbi summary :

This gene encodes a member of the glutathione peroxidase protein family. Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. A similar protein in rat has been identified as a moonlighting protein based on its ability to serve dual functions as a peroxidase as well as a structural protein in mature spermatozoa. Disruption of this gene in spermatocytes is associated with male infertility. Alternative splicing results in multiple transcript variants. Two pseudogenes of this gene have been defined on chromosomes 10 and 17. [provided by RefSeq, Jan 2014]

uniprot summary :

GPX4: Protects cells against membrane lipid peroxidation and cell death. Required for normal sperm development and male fertility. Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Belongs to the glutathione peroxidase family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Lipid Metabolism - arachidonic acid; Other Amino Acids Metabolism - glutathione; Mitochondrial; EC 1.11.1.12; Nuclear envelope; Oxidoreductase. Cellular Component: mitochondrion; cytoplasm; mitochondrial inner membrane; nuclear envelope; cytosol; nucleus. Molecular Function: selenium binding; peroxidase activity; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; oxidoreductase activity. Biological Process: establishment and/or maintenance of chromatin architecture; glutathione metabolic process; hydrogen peroxide catabolic process; multicellular organismal development; regulation of inflammatory response; spermatogenesis; response to oxidative stress

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage