Rat Rho-associated protein kinase 1, ROCK1 ELISA Kit | MyBioSource MBS909842 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Rho-associated protein kinase 1, ROCK1 ELISA Kit

catalog :

MBS909842

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909842

products type :

ELISA Kit

products full name :

Rat Rho-associated protein kinase 1, ROCK1 ELISA Kit

products short name :

Rho-associated, coiled-coil containing protein kinase 1

products name syn :

Rat Rho-associated protein kinase 1 (ROCK1) ELISA kit; MGC131603; MGC43611; P160ROCK; PRO0435; Rho kinase; p160-ROCK; renal carcinoma antigen NY-REN-35; Rho-associated; coiled-coil containing protein kinase 1

other names :

rho-associated protein kinase 1; Rho-associated protein kinase 1; rho-associated protein kinase 1; HEBM1; cePKA; p160ROCK; p160 ROCK-1; cAMP-dependent protein kinase ROCK-I; corneal epithelial Rho-associated-Ser/Thr kinase 1; rho-associated, coiled-coil-containing protein kinase 1; rho-associated, coiled-coil-containing protein kinase I; Corneal epithelial Rho-associated-Ser/Thr kinase 1; HEBM1; Rho-associated, coiled-coil-containing protein kinase 1; Rho-associated, coiled-coil-containing protein kinase I; ROCK-I; cAMP-dependent protein kinase ROCK-I; CePKA; p160 ROCK-1; p160ROCK

products gene name :

ROCK1

other gene names :

ROCK1; ROCK1; ROCK-I; ROCK-I; CePKA; p160ROCK

uniprot entry name :

ROCK1_RABIT

reactivity :

Rat

sequence length :

1354

specificity :

This assay has high sensitivity and excellent specificity for detection of rat ROCK1. No significant cross-reactivity or interference between rat ROCK1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, bronchoalveolar lavage fluid. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of rat ROCK1 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ROCK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ROCK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ROCK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ROCK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

126723142

ncbi acc num :

NP_001075836.1

ncbi gb acc num :

NM_001082367.1

uniprot acc num :

O77819

ncbi mol weight :

158,347 Da

uniprot summary :

Function: Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis Plays a role in terminal erythroid differentiation. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization 1 PublicationManual assertion based on experiment in:Ref.2. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium . Enzyme regulation: Activated by RHOA binding. Inhibited by Y-27632

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage