Human 14-3-3 protein sigma, SFN ELISA Kit | MyBioSource MBS909837 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 14-3-3 protein sigma, SFN ELISA Kit

catalog :

MBS909837

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909837

products type :

ELISA Kit

products full name :

Human 14-3-3 protein sigma, SFN ELISA Kit

products short name :

stratifin

products name syn :

Human 14-3-3 protein sigma (SFN) ELISA kit; YWHAS; 14-3-3 sigma; stratifin

other names :

14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 protein sigma; 14-3-3 sigma; epithelial cell marker protein 1; stratifin; Epithelial cell marker protein 1; Stratifin

products gene name :

SFN

other gene names :

SFN; SFN; YWHAS; HME1

uniprot entry name :

1433S_HUMAN

reactivity :

Human

sequence length :

248

specificity :

This assay has high sensitivity and excellent specificity for detection of human SFN. No significant cross-reactivity or interference between human SFN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of human SFN is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5454052

ncbi acc num :

NP_006133.1

ncbi gb acc num :

NM_006142.3

uniprot acc num :

P31947

ncbi mol weight :

27,774 Da

ncbi pathways :

Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020); Direct P53 Effectors Pathway (137939); ErbB1 Downstream Signaling Pathway (138057); FoxO Family Signaling Pathway (138036)

uniprot summary :

14-3-3 sigma: Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. Homodimer. Interacts with KRT17 and SAMSN1. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29, VPS35 and SFN. Interacts with GAB2. Interacts with SRPK2. Present mainly in tissues enriched in stratified squamous keratinizing epithelium. Belongs to the 14-3-3 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 1p36.11. Cellular Component: extracellular space; cytoplasmic vesicle membrane; cytoplasm; nucleus; cytosol. Molecular Function: protein domain specific binding; protein kinase C inhibitor activity; protein binding; phosphoprotein binding; protein kinase binding. Biological Process: positive regulation of protein export from nucleus; release of cytochrome c from mitochondria; regulation of epidermal cell division; DNA damage response, signal transduction resulting in induction of apoptosis; apoptosis; keratinization; positive regulation of epidermal cell differentiation; negative regulation of protein kinase activity; regulation of cyclin-dependent protein kinase activity; negative regulation of caspase activity; signal transduction; positive regulation of cell growth

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage