catalog number :
MBS909814
products type :
ELISA Kit
products full name :
Human Protein S100-A7A, S100A7A ELISA Kit
products short name :
S100 calcium binding protein A7A
products name syn :
Human Protein S100-A7A (S100A7A) ELISA kit; NICE-2; S100A15; S100A7L1; S100A7f; OTTHUMP00000015325; S100 calcium binding protein A15; S100 calcium binding protein A7-like 1; S100 calcium binding protein A7A
other names :
protein S100-A7A; Protein S100-A7A; protein S100-A7A; S100 calcium binding protein A15; S100 calcium-binding protein A15; S100 calcium-binding protein A7A; S100 calcium binding protein A7-like 1; S100 calcium-binding protein A7-like 1; S100 calcium binding protein A7A; S100 calcium-binding protein A15; S100 calcium-binding protein A7-like 1; S100 calcium-binding protein A7A
products gene name :
S100A7A
other gene names :
S100A7A; S100A7A; NICE-2; S100A15; S100A7f; S100A7L1; S100A15; S100A7L1
uniprot entry name :
S1A7A_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human S100A7A. No significant cross-reactivity or interference between human S100A7A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human S100A7A is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A7A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A7A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A7A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A7A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_789793.1
ncbi gb acc num :
NM_176823.3
ncbi mol weight :
11,305 Da
uniprot summary :
S100A7A: May be involved in epidermal differentiation and inflammation and might therefore be important for the pathogenesis of psoriasis and other diseases. Belongs to the S-100 family. Protein type: Cell development/differentiation. Chromosomal Location of Human Ortholog: 1q21.3. Cellular Component: cytoplasm. Molecular Function: calcium ion binding
size4 :
10x96-Strip-Wells