Mouse Protein HEXIM1, HEXIM1 ELISA Kit | MyBioSource MBS909626 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Protein HEXIM1, HEXIM1 ELISA Kit

catalog :

MBS909626

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909626

products type :

ELISA Kit

products full name :

Mouse Protein HEXIM1, HEXIM1 ELISA Kit

products short name :

hexamethylene bis-acetamide inducible 1

products name syn :

Mouse Protein HEXIM1 (HEXIM1) ELISA kit; CLP1; EDG1; FLJ13562; HIS1; MAQ1; HMBA-inducible; hexamethylene bisacetamide-inducible protein; hexamethylene-bis-acetamide-inducible transcript 1; hexamthylene bis-acetamide inducible 1; menage a quatr; hexamethylene bis-acetamide inducible 1

other names :

protein HEXIM1; Protein HEXIM1; protein HEXIM1; cardiac lineage protein 1; hexamethylene bis-acetamide inducible 1; Cardiac lineage protein 1

products gene name :

HEXIM1

other gene names :

Hexim1; Hexim1; Clp1; HIS1; CLP-1; 7330426E13Rik; Clp1

uniprot entry name :

HEXI1_MOUSE

reactivity :

Mouse

sequence length :

356

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse HEXIM1. No significant cross-reactivity or interference between mouse HEXIM1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: The minimum detectable dose of mouse HEXIM1 is typically less than 1.95 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HEXIM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HEXIM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HEXIM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HEXIM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

20270289

ncbi acc num :

NP_620092.1

ncbi gb acc num :

NM_138753.2

uniprot acc num :

Q8R409

ncbi mol weight :

40,243 Da

uniprot summary :

HEXIM1: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity. Homooligomer and heterooligomer with HEXIM2; probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with the N-CoR complex through NCOR1. Interacts with ESR1 and NR3C1. May interact with NF-kappa-B through RELA. Up-regulated by HMBA (hexamethylene bisacetamide). Down-regulated by estrogen. Ubiquitously expressed with higher expression in placenta. HEXIM1 and HEXIM2 are differentially expressed. Expressed in endocrine tissues. Belongs to the HEXIM family. Protein type: Motility/polarity/chemotaxis; Transcription factor. Cellular Component: nucleoplasm; cytoplasm; nucleus. Molecular Function: cyclin-dependent protein kinase inhibitor activity; protein binding; snRNA binding. Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; heart development; negative regulation of cyclin-dependent protein kinase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage