Human Tomoregulin-1, TMEFF1 ELISA Kit | MyBioSource MBS909548 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Tomoregulin-1, TMEFF1 ELISA Kit

catalog :

MBS909548

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909548

products type :

ELISA Kit

products full name :

Human Tomoregulin-1, TMEFF1 ELISA Kit

products short name :

transmembrane protein with EGF-like and two follistatin-like domains 1

products name syn :

Human Tomoregulin-1 (TMEFF1) ELISA kit; C9orf2; H7365; tomoregulin-1; transmembrane protein with EGF-like and two follistatin-like domains 1

other names :

MSANTD3-TMEFF1 fusion protein; Tomoregulin-1; MSANTD3-TMEFF1 fusion protein; MSANTD3-TMEFF1 readthrough; H7365; Transmembrane protein with EGF-like and one follistatin-like domain

products gene name :

TMEFF1

other gene names :

MSANTD3-TMEFF1; TMEFF1; C9orf3-TMEFF1; C9orf30-TMEFF1; C9orf2; TR-1

uniprot entry name :

TEFF1_HUMAN

reactivity :

Human

sequence length :

454

specificity :

This assay has high sensitivity and excellent specificity for detection of human TMEFF1. No significant cross-reactivity or interference between human TMEFF1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of human TMEFF1 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TMEFF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEFF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEFF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEFF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

311771742

ncbi acc num :

NP_001185741.1

ncbi gb acc num :

NM_001198812.1

uniprot acc num :

Q8IYR6

ncbi mol weight :

37,757 Da

ncbi summary :

This locus represents naturally occurring read-through transcription from the neighboring MSANTD3 (Myb/SANT-like DNA-binding domain containing 3) and TMEFF1 (transmembrane protein with EGF-like and two follistatin-like domains 1) genes. The read-through transcript encodes a fusion protein that shares sequence identity with the products of each individual gene. [provided by RefSeq, Aug 2013]

uniprot summary :

TMEFF1: a single-pass type I membrane protein. May inhibit NODAL and BMP signaling during neural patterning. A member of the Cancer-Testis Antigen (CTA) superfamily. CTAs may play roles in embryonal development and tumor transformation or aspects of tumor progression. CTAs were once thought to be silenced in most normal adult tissues, with limited expression in fetal, placental, testis, and ovarian cells. These proteins are now known to be aberrantly expressed in various cancers and many are capable of eliciting humoral and cellular immune responses. Expressed predominantly in brain, and at lower levels in heart, placenta and skeletal muscle. Down-regulated in brain tumors as compared to control brain tissues. Expressed in a variety of cancers cell lines and in myeloma cells. Belongs to the tomoregulin family. Two isoforms of the human protein are produced by alternative splicing. Note: This description may include information from RefSeq and UniProtKB. Protein type: Membrane protein, integral; Cancer Testis Antigen (CTA). Chromosomal Location of Human Ortholog: 9q31. Cellular Component: integral to membrane; plasma membrane. Biological Process: multicellular organismal development

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage