Human Keratin, type II cytoskeletal 1, KRT1 ELISA Kit | MyBioSource MBS909540 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Keratin, type II cytoskeletal 1, KRT1 ELISA Kit

catalog :

MBS909540

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909540

products type :

ELISA Kit

products full name :

Human Keratin, type II cytoskeletal 1, KRT1 ELISA Kit

products short name :

keratin 1

products name syn :

Human Keratin; type II cytoskeletal 1 (KRT1) ELISA kit; CK1; EHK; EHK1; EPPK; K1; KRT1A; NEPPK; cytokeratin 1; epidermolytic hyperkeratosis 1; hair alpha protein; keratin; type II cytoskeletal 1; keratin 1

other names :

keratin, type II cytoskeletal 1; Keratin, type II cytoskeletal 1; keratin, type II cytoskeletal 1; CK-1; cytokeratin 1; cytokeratin-1; 67 kDa cytokeratin; hair alpha protein; type-II keratin Kb1; epidermolytic hyperkeratosis 1; keratin 1; 67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1

products gene name :

KRT1

other gene names :

KRT1; KRT1; K1; CK1; EHK; EHK1; EPPK; KRT1A; NEPPK; KRTA; CK-1; K1

uniprot entry name :

K2C1_HUMAN

reactivity :

Human

sequence length :

644

specificity :

This assay has high sensitivity and excellent specificity for detection of Human KRT1. No significant cross-reactivity or interference between Human KRT1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

119395750

ncbi acc num :

NP_006112.3

ncbi gb acc num :

NM_006121.3

uniprot acc num :

P04264

ncbi mol weight :

66,039 Da

ncbi pathways :

Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway (169352)

ncbi summary :

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

uniprot summary :

K1: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K10. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13.13. Cellular Component: extracellular matrix; extracellular space; cytoskeleton; membrane; keratin filament; plasma membrane; nucleus. Molecular Function: protein binding; receptor activity; structural molecule activity; carbohydrate binding. Biological Process: retinal homeostasis; fibrinolysis; negative regulation of inflammatory response; response to oxidative stress; complement activation, lectin pathway; regulation of angiogenesis. Disease: Palmoplantar Keratoderma, Epidermolytic; Keratosis Palmoplantaris Striata Iii; Ichthyosis Hystrix, Curth-macklin Type; Palmoplantar Keratoderma, Nonepidermolytic; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage