catalog number :
MBS909493
products type :
ELISA Kit
products full name :
Mouse Delta-like protein 3, DLL3 ELISA Kit
products short name :
delta-like 3 (Drosophila)
products name syn :
Mouse Delta-like protein 3 (DLL3) ELISA kit; SCDO1; delta-like 3 protein; delta-like 3 (Drosophila)
other names :
delta-like protein 3; Delta-like protein 3; delta-like protein 3; delta3; M-Delta-3; drosophila Delta homolog 3; delta-like 3 (Drosophila); Drosophila Delta homolog 3; Delta3; M-Delta-3
products gene name :
DLL3
other gene names :
Dll3; Dll3; pu; pudgy; Delta3; M-Delta-3
uniprot entry name :
DLL3_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse DLL3. No significant cross-reactivity or interference between Mouse DLL3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DLL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DLL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031892.2
ncbi gb acc num :
NM_007866.2
ncbi mol weight :
61,125 Da
ncbi pathways :
Notch Signaling Pathway (198301); Notch Signaling Pathway (83259); Notch Signaling Pathway (473)
uniprot summary :
DLL3: Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm. Can bind and activate Notch-1 or another Notch receptor. Protein type: Membrane protein, integral; Cell development/differentiation. Cellular Component: membrane; integral to membrane. Molecular Function: protein binding; Notch binding. Biological Process: paraxial mesoderm development; nervous system development; tissue development; Notch signaling pathway; somitogenesis; positive regulation of neurogenesis; in utero embryonic development; multicellular organismal development; cell fate determination; negative regulation of neurogenesis; compartment specification; negative regulation of astrocyte differentiation; negative regulation of Notch signaling pathway; cell differentiation; skeletal development
size4 :
10x96-Strip-Wells