product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Gastric intrinsic factor, GIF ELISA Kit
catalog :
MBS909449
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS909449
products type :
ELISA Kit
products full name :
Rat Gastric intrinsic factor, GIF ELISA Kit
products short name :
gastric intrinsic factor (vitamin B synthesis)
products name syn :
Rat Gastric intrinsic factor (GIF) ELISA kit; IF; IFMH; INF; TCN3; ; gastric intrinsic factor (vitamin B synthesis)
other names :
gastric intrinsic factor; Gastric intrinsic factor; gastric intrinsic factor; IF; INF; gastric intrinsic factor; Intrinsic factor; IF; INF
products gene name :
GIF
other gene names :
Gif; Gif; IF; INF
uniprot entry name :
IF_RAT
reactivity :
Rat
sequence length :
421
specificity :
This assay has high sensitivity and excellent specificity for detection of rat GIF. No significant cross-reactivity or interference between rat GIF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 6.25 mU/ml - 400 mU/ml. Sensitivity: 1.56 mU/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the direct competitive inhibition enzyme immunoassay technique. Antibody specific for GIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells with biotin-conjugated GIF. A competitive inhibition reaction is launched between GIF (Standards or samples) and biotin-conjugated GIF with the pre-coated GIF antibody. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of GIF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
8393437
ncbi acc num :
NP_058858.1
ncbi gb acc num :
NM_017162.3
uniprot acc num :
P17267
ncbi mol weight :
45,956 Da
ncbi pathways :
Vitamin Digestion And Absorption Pathway (199553); Vitamin Digestion And Absorption Pathway (199538)
ncbi summary :
binds vitamin B12 (cobalamin) [RGD, Feb 2006]
uniprot summary :
GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular space; microvillus; apical plasma membrane; endosome. Molecular Function: vitamin binding; cobalamin binding. Biological Process: cobalamin metabolic process; cobalamin transport; cobalt ion transport
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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