Mouse Lysyl oxidase homolog 2, LOXL2 ELISA Kit | MyBioSource MBS909320 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Lysyl oxidase homolog 2, LOXL2 ELISA Kit

catalog :

MBS909320

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909320

products type :

ELISA Kit

products full name :

Mouse Lysyl oxidase homolog 2, LOXL2 ELISA Kit

products short name :

lysyl oxidase-like 2

products name syn :

Mouse Lysyl oxidase homolog 2 (LOXL2) ELISA kit; LOR2; WS9-14; lysyl oxidase homolog 2; lysyl oxidase related 2; lysyl oxidase-like 2

other names :

lysyl oxidase homolog 2; Lysyl oxidase homolog 2; lysyl oxidase homolog 2; lysyl oxidase-like protein 2; lysyl oxidase-like 2; Lysyl oxidase-like protein 2

products gene name :

LOXL2

other gene names :

Loxl2; Loxl2; 1110004B06Rik; 4930526G11Rik; 9430067E15Rik

uniprot entry name :

LOXL2_MOUSE

reactivity :

Mouse

sequence length :

776

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse LOXL2. No significant cross-reactivity or interference between mouse LOXL2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LOXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LOXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXL2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

225579028

ncbi acc num :

NP_201582.2

ncbi gb acc num :

NM_033325.2

uniprot acc num :

P58022

ncbi mol weight :

87,003 Da

uniprot summary :

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated Lys-4 of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family. Protein type: Oxidoreductase; Secreted, signal peptide; Cell adhesion; Secreted; EC 1.4.3.13. Cellular Component: nucleoplasm; extracellular space; basement membrane; nucleus. Molecular Function: protein-lysine 6-oxidase activity; chromatin binding; transcription corepressor activity; methylated histone residue binding. Biological Process: collagen fibril organization; response to copper ion; endothelial cell proliferation; histone modification; protein modification process; epithelial to mesenchymal transition; endothelial cell migration; sprouting angiogenesis; negative regulation of transcription, DNA-dependent; protein amino acid deamination

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage