ELISA/assay

Rat C-C motif chemokine 6, CCL6 ELISA Kit

MBS909316

96 Strip Wells

760 USD

ELISA Kit

Rat C-C motif chemokine 6, CCL6 ELISA Kit

C-C motif chemokine 6 (CCL6)

Rat C-C motif chemokine 6 (CCL6) ELISA kit; Small-inducible cytokine A6; C-C motif chemokine 6 (CCL6)

C-C motif chemokine 6; C-C motif chemokine 6; C-C motif chemokine 6; CC chemokine C10; small inducible cytokine A6; small-inducible cytokine A6; chemokine (C-C motif) ligand 6; Protein C10; Small-inducible cytokine A6Cleaved into the following 2 chains:CCL6(22-95); CCL6(23-95)

CCL6

Ccl6; Ccl6; c10; MRP-1; Scya6; C10; Scya6

CCL6_MOUSE

Rat

116

This assay has high sensitivity and excellent specificity for detection of rat CCL6. No significant cross-reactivity or interference between rat CCL6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of rat C-C motif chemokine 6 (CCL6) concentrations in serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.156 ng/ml -10 ng/ml.

Sensitivity: The minimum detectable dose of rat CCL6 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CCL6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CCL6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6677883

NP_033165.1

NM_009139.3

P27784

12,984 Da

Chemokine Signaling Pathway 99271!!Chemokine Signaling Pathway 96864!!Class A/1 (Rhodopsin-like Receptors) Pathway 575426!!Cytokine-cytokine Receptor Interaction Pathway 83248!!Cytokine-cytokine Receptor Interaction Pathway 460!!Formyl Peptide Receptors Bind Formyl Peptides And Many Other Ligands Pathway 575432!!G Alpha (i) Signalling Events Pathway 575454!!G Alpha (q) Signalling Events Pathway 575456!!GPCR Downstream Signaling Pathway 575452!!GPCR Ligand Binding Pathway 575425

Function: CCL6(22-95) and CCL6(23-95) are potent chemoattractants.1 PublicationManual assertion based on experiment in:Ref.7

96 Strip Wells

760 USD