catalog number :
MBS909315
products type :
ELISA Kit
products full name :
Mouse Osteocrin, OSTN ELISA Kit
products short name :
osteocrin
products name syn :
Mouse Osteocrin (OSTN) ELISA kit; MUSCLIN; ; osteocrin
other names :
osteocrin; Osteocrin; osteocrin; musclin; osteocrin; Musclin
products gene name :
OSTN
other gene names :
Ostn; Ostn; Ostc
uniprot entry name :
OSTN_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse OSTN. No significant cross-reactivity or interference between mouse OSTN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: 3.9 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_932780.1
ncbi gb acc num :
NM_198112.2
ncbi mol weight :
14,438 Da
uniprot summary :
OSTN: Appears to modulate osteoblastic differentiation. Could also function as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular space; extracellular region. Molecular Function: hormone activity; receptor binding. Biological Process: ossification; negative regulation of glucose import; cell surface receptor linked signal transduction; positive regulation of cGMP biosynthetic process; multicellular organismal development; negative regulation of osteoblast differentiation; cell differentiation; regulation of bone mineralization
size4 :
10x96-Strip-Wells