ELISA/assay

Human Kallistatin, SERPINA4 ELISA Kit

MBS909102

96-Strip-Wells

915 USD

ELISA Kit

Human Kallistatin, SERPINA4 ELISA Kit

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) , member 4

Human Kallistatin (SERPINA4) ELISA kit; KAL; KLST; KST; PI4; kallistatin; protease inhibitor 4 (kallistatin) ; serine (or cysteine) proteinase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 4; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 4

kallistatin isoform 1; Kallistatin; kallistatin; serpin A4; kallikrein inhibitor; peptidase inhibitor 4; protease inhibitor 4 (kallistatin); serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 4; Kallikrein inhibitor; Peptidase inhibitor 4; PI-4; Serpin A4

SERPINA4

SERPINA4; SERPINA4; KAL; KST; PI4; KLST; PI-4; kallistatin; KST; PI4; PI-4

KAIN_HUMAN

Human

464

This assay has high sensitivity and excellent specificity for detection of human SERPINA4. No significant cross-reactivity or interference between human SERPINA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human SERPINA4 is typically less than 0.039 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SERPINA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SERPINA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

573459706

NP_001275961.1

NM_001289032.1

P29622

48,542 Da

SERPINA4: Inhibits human amidolytic and kininogenase activities of tissue kallikrein. Inhibition is achieved by formation of an equimolar, heat- and SDS-stable complex between the inhibitor and the enzyme, and generation of a small C-terminal fragment of the inhibitor due to cleavage at the reactive site by tissue kallikrein. Belongs to the serpin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 14q32.13. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity

96-Strip-Wells

915 USD

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800