ELISA/assay

Rat Glycogen synthase kinase-3 beta, GSK3B ELISA Kit

MBS909078

48-Strip-Wells

510 USD

ELISA Kit

Rat Glycogen synthase kinase-3 beta, GSK3B ELISA Kit

glycogen synthase kinase 3 beta

Rat Glycogen synthase kinase-3 beta (GSK3B) ELISA kit; GSK3beta isoform; glycogen synthase kinase 3 beta

glycogen synthase kinase-3 beta; Glycogen synthase kinase-3 beta; glycogen synthase kinase-3 beta; FA; factor A; GSK-3 beta; serine/threonine-protein kinase GSK3B; glycogen synthase kinase 3 beta; Factor A; FA; Serine/threonine-protein kinase GSK3B (EC:2.7.11.1)

GSK3B

Gsk3b; Gsk3b; GSK-3 beta; FA

GSK3B_RAT

Rat

420

This assay has high sensitivity and excellent specificity for detection of rat GSK3B. No significant cross-reactivity or interference between rat GSK3B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat GSK3B is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GSK3B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GSK3B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GSK3B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GSK3B bound in the initial step. The color development is stopped and the intensity of the color is measured.

14091770

NP_114469.1

NM_032080.1

P18266

46,742 Da

AKT Phosphorylates Targets In The Cytosol Pathway (573650); Adaptive Immune System Pathway (573825); Alzheimer's Disease Pathway (83489); Alzheimer's Disease Pathway (509); Axon Guidance Pathway (83457); Axon Guidance Pathway (476); Axon Guidance Pathway (574273); B Cell Receptor Signaling Pathway (198486); B Cell Receptor Signaling Pathway (83473); B Cell Receptor Signaling Pathway (492)

mediates Par6-atypical protein kinase C (aPKC) complex regulation of cell polarity; may induce apoptosis [RGD, Feb 2006]

GSK3B: a proline-directed protein kinase of the GSK family. Phosphorylates and inactivates glycogen synthase. Participates in the Wnt signaling pathway. Involved in energy metabolism, neuronal cell development, and body pattern formation. Protein type: EC 2.7.11.1; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.26; CMGC group; GSK family; GSK subfamily. Cellular Component: centrosome; protein complex; dendritic spine; ribonucleoprotein complex; beta-catenin destruction complex; cytosol; lipid raft; nucleoplasm; growth cone; cell soma; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; dendritic shaft; nucleus. Molecular Function: integrin binding; NF-kappaB binding; ionotropic glutamate receptor binding; protein serine/threonine kinase activity; protein binding; p53 binding; ubiquitin protein ligase binding; tau-protein kinase activity; beta-catenin binding; kinase activity; tau protein binding; transcription factor binding; protein kinase binding; ATP binding; protein kinase activity. Biological Process: glycogen metabolic process; fat cell differentiation; circadian rhythm; negative regulation of MAP kinase activity; re-entry into mitotic cell cycle; genetic imprinting; positive regulation of protein binding; positive regulation of apoptosis; response to lithium ion; protein amino acid autophosphorylation; positive regulation of axon extension; establishment and/or maintenance of cell polarity; Wnt receptor signaling pathway through beta-catenin; protein amino acid phosphorylation; positive regulation of protein export from nucleus; protein export from nucleus; positive regulation of cell-matrix adhesion; negative regulation of protein binding; ER overload response; myoblast fusion; response to drug; regulation of microtubule-based process; cell migration; Wnt receptor signaling pathway; hippocampus development; myotube differentiation; negative regulation of dendrite morphogenesis; negative regulation of NFAT protein import into nucleus; positive regulation of peptidyl-serine phosphorylation; organ morphogenesis; peptidyl-serine phosphorylation; negative regulation of neuron maturation; positive regulation of protein complex assembly; axonogenesis; establishment of cell polarity; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; epithelial to mesenchymal transition; positive regulation of transcription from RNA polymerase II promoter; phosphorylation; negative regulation of protein complex assembly; negative regulation of apoptosis; regulation of neuronal synaptic plasticity

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800