ELISA/assay

Human Trefoil factor 2, TFF2 ELISA Kit

MBS909060

48-Strip-Wells

510 USD

ELISA Kit

Human Trefoil factor 2, TFF2 ELISA Kit

trefoil factor 2

Human Trefoil factor 2 (TFF2) ELISA kit; SML1; SP; spasmolysin; spasmolytic polypeptide; spasmolytic protein 1; trefoil factor 2

trefoil factor 2; Trefoil factor 2; trefoil factor 2; spasmolysin; spasmolytic protein 1; spasmolytic polypeptide; trefoil factor 2; Spasmolysin; Spasmolytic polypeptide; SP

TFF2

TFF2; TFF2; SP; SML1; SML1; SP

TFF2_HUMAN

Human

129

This assay has high sensitivity and excellent specificity for detection of human TFF2. No significant cross-reactivity or interference between human TFF2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: 7.8 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TFF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TFF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TFF2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TFF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4885629

NP_005414.1

NM_005423.4

Q03403

14,284 Da

Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. The encoded protein inhibits gastric acid secretion. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008]

TFF2: Inhibits gastrointestinal motility and gastric acid secretion. Could function as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: extracellular space. Molecular Function: protein binding; CXCR4 chemokine receptor binding. Biological Process: negative regulation of inflammatory response; positive regulation of cell proliferation; digestion; calcium-mediated signaling; negative regulation of macrophage activation; regulation of cell migration

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800