Human DNA repliCation licensing factor MCM6, MCM6 ELISA Kit | MyBioSource MBS909031 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human DNA repliCation licensing factor MCM6, MCM6 ELISA Kit

catalog :

MBS909031

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909031

products type :

ELISA Kit

products full name :

Human DNA repliCation licensing factor MCM6, MCM6 ELISA Kit

products short name :

minichromosome maintenance complex component 6

products name syn :

Human DNA replication licensing factor MCM6 (MCM6) ELISA kit; MCG40308; Mis5; P105MCM; DNA replication licensing factor MCM6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog; S. pombe) ; MIS5 homolog; minichromosome maintenance deficient (mis5; S. pombe) ; minichromosome maintenance complex component 6

other names :

DNA replication licensing factor MCM6; DNA replication licensing factor MCM6; DNA replication licensing factor MCM6; minichromosome maintenance deficient (mis5, S. pombe) 6; MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe); minichromosome maintenance complex component 6; p105MCM

products gene name :

MCM6

other gene names :

MCM6; MCM6; Mis5; P105MCM; MCG40308

uniprot entry name :

MCM6_HUMAN

reactivity :

Human

sequence length :

821

specificity :

This assay has high sensitivity and excellent specificity for detection of Human MCM6. No significant cross-reactivity or interference between Human MCM6 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml-3000 pg/ml. Sensitivity: 11.72 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Cycle. Protein Biological Process 3: Cell cycle

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MCM6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MCM6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MCM6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MCM6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

7427519

ncbi acc num :

NP_005906.2

ncbi gb acc num :

NM_005915.5

uniprot acc num :

Q14566

ncbi mol weight :

92,889 Da

ncbi pathways :

Activation Of ATR In Response To Replication Stress Pathway (105754); Activation Of The Pre-replicative Complex Pathway (105777); Assembly Of The Pre-replicative Complex Pathway (105821); Cell Cycle Pathway (530733); Cell Cycle Checkpoints Pathway (105739); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); DNA Replication Pathway (198771)

ncbi summary :

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. Single nucleotide polymorphisms in the intron regions of this gene are associated with differential transcriptional activation of the promoter of the neighboring lactase gene and, thereby, influence lactose intolerance in early adulthood. [provided by RefSeq, May 2012]

uniprot summary :

MCM6: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for once per cell cycle DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Belongs to the MCM family. Protein type: EC 3.6.4.12; Cell cycle regulation. Chromosomal Location of Human Ortholog: 2q21. Cellular Component: nucleoplasm; MCM complex; nucleus. Molecular Function: ATP-dependent DNA helicase activity; identical protein binding; protein binding; ATP binding; single-stranded DNA binding. Biological Process: DNA unwinding during replication; DNA replication initiation; mitotic cell cycle; DNA strand elongation during DNA replication; DNA replication; G1/S transition of mitotic cell cycle. Disease: Lactose Intolerance, Adult Type

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage