Human G protein-coupled receptor kinase 5, GRK5 ELISA Kit | MyBioSource MBS909001 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human G protein-coupled receptor kinase 5, GRK5 ELISA Kit

catalog :

MBS909001

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS909001

products type :

ELISA Kit

products full name :

Human G protein-coupled receptor kinase 5, GRK5 ELISA Kit

products short name :

G protein-coupled receptor kinase 5

products name syn :

Human G protein-coupled receptor kinase 5 (GRK5) ELISA kit; GPRK5; ; G protein-coupled receptor kinase 5

other names :

G protein-coupled receptor kinase 5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase 5; FP2025; g protein-coupled receptor kinase GRK5; G protein-coupled receptor kinase 5; G protein-coupled receptor kinase GRK5

products gene name :

GRK5

other gene names :

GRK5; GRK5; GPRK5; GPRK5

uniprot entry name :

GRK5_HUMAN

reactivity :

Human

sequence length :

590

specificity :

This assay has high sensitivity and excellent specificity for detection of human GRK5. No significant cross-reactivity or interference between human GRK5 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 28 pg/ml-1800 pg/ml. Sensitivity: Typically less than 7 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRK5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRK5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRK5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRK5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4885349

ncbi acc num :

NP_005299.1

ncbi gb acc num :

NM_005308.2

uniprot acc num :

P34947

ncbi mol weight :

67,787 Da

ncbi pathways :

Alpha-synuclein Signaling Pathway (137913); Calcium Regulation In The Cardiac Cell Pathway (198906); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Endocytosis Pathway (102279); Endocytosis Pathway (102181); G Alpha (q) Signalling Events Pathway (106043); GPCR Downstream Signaling Pathway (119548); Morphine Addiction Pathway (552665); Morphine Addiction Pathway (554808)

ncbi summary :

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating their deactivation. It has also been shown to play a role in regulating the motility of polymorphonuclear leukocytes (PMNs). [provided by RefSeq, Jul 2008]

uniprot summary :

GRK5: Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein- coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors. In addition to GPCRs, also phosphorylates various substrates: Hsc70- interacting protein/ST13, TP53/p53, HDAC5, and arrestin-1/ARRB1. Phosphorylation of ARRB1 by GRK5 inhibits G-protein independent MAPK1/MAPK3 signaling downstream of 5HT4-receptors. Phosphorylation of HDAC5, a repressor of myocyte enhancer factor 2 (MEF2) leading to nuclear export of HDAC5 and allowing MEF2- mediated transcription. Phosphorylation of TP53/p53, a crucial tumor suppressor, inhibits TP53/p53-mediated apoptosis. Phosphorylation of ST13 regulates internalization of the chemokine receptor. Phosphorylates rhodopsin (RHO) (in vitro) and a non G- protein-coupled receptor, LRP6 during Wnt signaling (in vitro). Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Protein type: EC 2.7.11.16; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; GRK family; GRK subfamily. Chromosomal Location of Human Ortholog: 10q26.11. Cellular Component: nucleoplasm; nuclear membrane; focal adhesion; cytoplasm; plasma membrane; nucleus. Molecular Function: G-protein coupled receptor kinase activity; protein serine/threonine kinase activity; protein binding; protein kinase C binding; phospholipid binding; ATP binding. Biological Process: G-protein signaling, coupled to cAMP nucleotide second messenger; tachykinin signaling pathway; Wnt receptor signaling pathway; apoptosis; protein amino acid autophosphorylation; regulation of G-protein coupled receptor protein signaling pathway; negative regulation of apoptosis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage