ELISA/assay

Bovine Acetylcholinesterase, ACHE ELISA Kit

MBS908990

96 Strip Wells

850 USD

ELISA Kit

Bovine Acetylcholinesterase, ACHE ELISA Kit

acetylcholinesterase (Yt blood group)

Bovine Acetylcholinesterase (ACHE) ELISA kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)

acetylcholinesterase; Acetylcholinesterase; acetylcholinesterase; acetylcholinesterase (Yt blood group); acetylcholinesterase<

ACHE

ACHE; ACHE; AChE

ACES_BOVIN

Bovine

613

This assay has high sensitivity and excellent specificity for detection of bovine ACHE. No significant cross-reactivity or interference between bovine ACHE and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of bovine acetylcholinesterase (ACHE) concentrations in serum, plasma. Detection Range: 0.9 4 U/ml -60 U/ml.

Sensitivity: The minimum detectable dose of bovine ACHE is typically less than 0.24 U/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ACHE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ACHE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ACHE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ACHE bound in the initial step. The color development is stopped and the intensity of the color is measured.

115497516

NP_001069688.1

NM_001076220.1

P23795

67,664 Da

Acetylcholine Synthesis Pathway 198547!!Cholinergic Synapse Pathway 217715!!Glycerophospholipid Metabolism Pathway 84232!!Glycerophospholipid Metabolism Pathway 364!!Neuronal System Pathway 582047!!Neurotransmitter Clearance In The Synaptic Cleft Pathway 582060!!Transmission Across Chemical Synapses Pathway 582050

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Catalytic activity: Acetylcholine + H2O = choline + acetate.

96 Strip Wells

850 USD