catalog number :
MBS908981
products type :
ELISA Kit
products full name :
Human Cerebellin-4, CBLN4 ELISA Kit
products short name :
cerebellin 4 precursor
products name syn :
Human Cerebellin-4 (CBLN4) ELISA kit; CBLNL1; cerebellin 4; cerebellin precursor-like 1; cerebellin-like glycoprotein 1; cerebellin 4 precursor
other names :
cerebellin-4; Cerebellin-4; cerebellin-4; cerebellin precursor-like 1; cerebellin-like glycoprotein 1; cerebellin 4 precursor; Cerebellin-like glycoprotein 1
products gene name :
CBLN4
other gene names :
CBLN4; CBLN4; CBLNL1; CBLNL1; UNQ718/PRO1382
uniprot entry name :
CBLN4_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CBLN4. No significant cross-reactivity or interference between human CBLN4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: The minimum detectable dose of human CBLN4 is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CBLN4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CBLN4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CBLN4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CBLN4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_542184.1
ncbi gb acc num :
NM_080617.5
ncbi mol weight :
21,808 Da
ncbi summary :
This gene encodes a member of a family of small secreted proteins containing C1Q domains. Members of this family are involved in regulation of neurexin signalling during synapse development. The mouse homolog of the protein encoded by this gene competes with netrin to bind to the deleted in colorectal cancer receptor. [provided by RefSeq, Aug 2012]
uniprot summary :
CBLN4: May be involved in synaptic functions in the CNS. Can enable ER export and secretion of CBLN3. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 20q13. Cellular Component: extracellular space; synapse; cell junction. Biological Process: protein secretion
size4 :
10x96-Strip-Wells