catalog number :
MBS908928
products type :
ELISA Kit
products full name :
Rat UDP-glucuronosyltransferase 2B7, UGT2B7 ELISA Kit
products short name :
UDP glucuronosyltransferase 2 family, polypeptide B7
products name syn :
Rat UDP-glucuronosyltransferase 2B7 (UGT2B7) ELISA kit; UDPGT2B7; UDPGTH2; UGT2B9; 3; 4-catechol estrogen specific; UDP glucuronosyltransferase 2B7; UDP-glucuronosyltransferase 2B7; UDP-glucuronyltransferase; family 2; beta-7; UDP glucuronosyltransferase 2 family; polypeptide B7
other names :
UDP-glucuronosyltransferase 2B7; UDP-glucuronosyltransferase 2B7; UDP-glucuronosyltransferase 2B7; UDPGT 2B8; UGT2B-RH4; UDP-glucuronosyltransferase 2B8; UDP glucuronosyltransferase 2 family, polypeptide B7; UGT2B-RH4
products gene name :
UGT2B7
other gene names :
Ugt2b7; Ugt2b7; Ugt2b8; UDPGT 2B7; Ugt2b8; UDPGT 2B7
uniprot entry name :
UD2B7_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat UGT2B7. No significant cross-reactivity or interference between Rat UGT2B7 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml-1200 pg/ml. Sensitivity: 4.69 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UGT2B7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UGT2B7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UGT2B7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UGT2B7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_775445.1
ncbi gb acc num :
NM_173323.1
ncbi mol weight :
60,089 Da
ncbi pathways :
Glucuronidation Pathway (219781)
uniprot summary :
UGT2B4: UDPGTs are of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isozyme is active on polyhydroxylated estrogens (such as estriol, 4-hydroxyestrone and 2-hydroxyestriol) and xenobiotics (such as 4-methylumbelliferone, 1-naphthol, 4- nitrophenol, 2-aminophenol, 4-hydroxybiphenyl and menthol). It is capable of 6 alpha-hydroxyglucuronidation of hyodeoxycholic acid. Belongs to the UDP-glycosyltransferase family. Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 2.4.1.17; Transferase; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - ascorbate and aldarate; Lipid Metabolism - androgen and estrogen; Cofactor and Vitamin Metabolism - retinol; Membrane protein, integral; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Carbohydrate Metabolism - starch and sucrose. Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; integral to membrane. Molecular Function: retinoic acid binding; glucuronosyltransferase activity. Biological Process: flavonoid biosynthetic process; androgen metabolic process; cellular response to hormone stimulus
size4 :
10x96-Strip-Wells