ELISA/assay

Mouse Vasodilator-stimulated phosphoprotein, VASP ELISA Kit

MBS908839

96 Strip Wells

760 USD

ELISA Kit

Mouse Vasodilator-stimulated phosphoprotein, VASP ELISA Kit

vasodilator-stimulated phosphoprotein

Mouse Vasodilator-stimulated phosphoprotein (VASP) ELISA kit; vasodilator-stimulated phosphoprotein

vasodilator-stimulated phosphoprotein isoform 2; Vasodilator-stimulated phosphoprotein; vasodilator-stimulated phosphoprotein; vasodilator-stimulated phosphoprotein

VASP

Vasp; Vasp; VASP

VASP_MOUSE

Mouse

374

This assay has high sensitivity and excellent specificity for detection of mouse VASP. No significant cross-reactivity or interference between mouse VASP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of mouse vasodilator-stimulated phosphoprotein (VASP) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 6.25 pg/ml -400 pg/ml.

Sensitivity: The minimum detectable dose of mouse VASP is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VASP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VASP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASP bound in the initial step. The color development is stopped and the intensity of the color is measured.

530537260

NP_001268950.1

NM_001282021.1

P70460

39,667 Da

Adaptive Immune System Pathway 575096!!Axon Guidance Pathway 575211!!Developmental Biology Pathway 575210!!Fc Gamma R-mediated Phagocytosis Pathway 114297!!Fc Gamma R-mediated Phagocytosis Pathway 106590!!Focal Adhesion Pathway 198353!!Focal Adhesion Pathway 83264!!Focal Adhesion Pathway 478!!Generation Of Second Messenger Molecules Pathway 575100!!Immune System Pathway 575095

Function: Ena/VASP proteins are actin-associated proteins involved in a range of processes dependent on cytoskeleton remodeling and cell polarity such as axon guidance, lamellipodial and filopodial dynamics, platelet activation and cell migration. VASP promotes actin filament elongation. It protects the barbed end of growing actin filaments against capping and increases the rate of actin polymerization in the presence of capping protein. VASP stimulates actin filament elongation by promoting the transfer of profilin-bound actin monomers onto the barbed end of growing actin filaments. Plays a role in actin-based mobility of Listeria monocytogenes in host cells. Regulates actin dynamics in platelets and plays an important role in regulating platelet aggregation 1 PublicationManual assertion based on experiment in:Ref.6

96 Strip Wells

760 USD