Rat G protein-coupled receptor kinase 6, GRK6 ELISA Kit | MyBioSource MBS908808 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat G protein-coupled receptor kinase 6, GRK6 ELISA Kit

catalog :

MBS908808

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS908808

products type :

ELISA Kit

products full name :

Rat G protein-coupled receptor kinase 6, GRK6 ELISA Kit

products short name :

[G protein-coupled receptor kinase 6]

products name syn :

[Rat G protein-coupled receptor kinase 6 (GRK6) ELISA kit; FLJ32135; GPRK6; ; G protein-coupled receptor kinase 6]

other names :

[G protein-coupled receptor kinase 6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase 6; G-protein coupled receptor kinase 6; g protein-coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6]

products gene name :

[GRK6]

other gene names :

[Grk6; Grk6; Gprk6; Gprk6]

uniprot entry name :

GRK6_RAT

reactivity :

Rat

sequence length :

576

specificity :

This assay has high sensitivity and excellent specificity for detection of rat GRK6. No significant cross-reactivity or interference between rat GRK6 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GRK6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GRK6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRK6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRK6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

3023902

ncbi acc num :

P97711.1

uniprot acc num :

P97711

ncbi mol weight :

65,963 Da

ncbi pathways :

Calcium Regulation In The Cardiac Cell Pathway (198495); Chemokine Signaling Pathway (98755); Chemokine Signaling Pathway (96864); Endocytosis Pathway (102267); Endocytosis Pathway (102181); Morphine Addiction Pathway (552694); Morphine Addiction Pathway (554808); Myometrial Relaxation And Contraction Pathways (198465)

ncbi summary :

G-protein coupled receptor kinase that desensitizes beta adrenergic receptors in myometrium [RGD, Feb 2006]

uniprot summary :

GRK6: Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamin receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro). Widely expressed. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.16; Protein kinase, AGC; Kinase, protein; AGC group; GRK family; GRK subfamily. Cellular Component: membrane; plasma membrane; cytosol. Molecular Function: G-protein coupled receptor kinase activity; ATP binding. Biological Process: desensitization of G-protein coupled receptor protein signaling pathway; Wnt receptor signaling pathway; signal transduction; protein amino acid phosphorylation; negative regulation of anion channel activity

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145