ELISA/assay

Rat Protein NOV homolog, NOV ELISA Kit

MBS908721

48-Strip-Wells

510 USD

ELISA Kit

Rat Protein NOV homolog, NOV ELISA Kit

nephroblastoma overexpressed gene

Rat Protein NOV homolog (NOV) ELISA kit; CCN3; IGFBP9; nephroblastoma overexpressed; nephroblastoma overexpressed gene

protein NOV homolog; Protein NOV homolog; protein NOV homolog; novH; CCN family member 3; nephroblastoma overexpressed gene protein homolog; nephroblastoma-overexpressed gene protein homolog; nephroblastoma overexpressed; CCN family member 3; Nephroblastoma-overexpressed gene protein homolog

NOV

Nov; Nov; Ccn3; NovH

NOV_RAT

Rat

351

This assay has high sensitivity and excellent specificity for detection of rat NOV. No significant cross-reactivity or interference between rat NOV and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 ng/ml -2000 ng/ml. Sensitivity: The minimum detectable dose of rat NOV is typically less than 7.81 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NOV has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NOV present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOV is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOV bound in the initial step. The color development is stopped and the intensity of the color is measured.

13540697

NP_110495.1

NM_030868.2

Q9QZQ5

38,510 Da

Delta-Notch Signaling Pathway (198480)

acts as a growth factor for some cells and binds to a specific receptor; may also have growth suppressive effects [RGD, Feb 2006]

NOV: Immediate-early protein likely to play a role in cell growth regulation. Belongs to the CCN family. Expression is down-regulated by WT1. Interacts with FBLN1. Protein type: Secreted, signal peptide; Secreted. Cellular Component: extracellular matrix; proteinaceous extracellular matrix; cell soma; intracellular membrane-bound organelle; axon; dendrite. Molecular Function: heparin binding; integrin binding; insulin-like growth factor binding; growth factor activity. Biological Process: cell-cell signaling; regulation of gene expression; regulation of cell growth; signal transduction; cell adhesion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800