Rat 5'-nucleotidase/cluster of differentiation 73, NT5E/CD73 ELISA Kit | MyBioSource MBS908567 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat 5'-nucleotidase/cluster of differentiation 73, NT5E/CD73 ELISA Kit

catalog :

MBS908567

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS908567

products type :

ELISA Kit

products full name :

Rat 5'-nucleotidase/cluster of differentiation 73, NT5E/CD73 ELISA Kit

products short name :

5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73)

products name syn :

Rat 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73) ELISA kit; 5'-nucleotidase/cluster of differentiation 73 (NT5E/CD73)

other names :

NT5E protein; 5'-nucleotidase; 5'-nucleotidase; 5'-NT; ecto-5'-nucleotidase; Purine 5-Prime-Nucleotidase; 5'-nucleotidase, ecto (CD73); 5'-nucleotidase; 5'-nucleotidase, ecto (CD73), isoform CRA_c; NT5E protein

products gene name :

NT5E/CD73

other gene names :

NT5E; NT5E; NT; eN; NT5; NTE; eNT; CD73; E5NT; CALJA; hCG_401111

uniprot entry name :

Q96B60_HUMAN

reactivity :

Rat

sequence length :

264

specificity :

This assay has high sensitivity and excellent specificity for detection of Rat NT5E/CD73. No significant cross-reactivity or interference between Rat NT5E/CD73 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and cell culture supernates. Assay Type: Sandwich. Detection Range: 0.625 mIU/ml-40 mIU/ml. Sensitivity: 0.156 mIU/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CD73 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CD73 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CD73 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CD73 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

16198518

ncbi acc num :

AAH15940.1

uniprot acc num :

Q96B60

ncbi mol weight :

28,841 Da

ncbi pathways :

HIF-1-alpha Transcription Factor Network Pathway (138045); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Nicotinate And Nicotinamide Metabolism Pathway (83014); Nicotinate And Nicotinamide Metabolism Pathway (400); Purine Catabolism Pathway (106276); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Pyrimidine Catabolism Pathway (106288)

ncbi summary :

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

uniprot summary :

NT5E: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Homodimer; disulfide-linked. Belongs to the 5 -nucleotidase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleotide Metabolism - purine; Membrane protein, integral; EC 3.1.3.5; Nucleotide Metabolism - pyrimidine; Membrane protein, GPI anchor; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein). Chromosomal Location of Human Ortholog: 6q14-q21. Cellular Component: cell surface; membrane; cytoplasm; plasma membrane. Molecular Function: 5 -nucleotidase activity; metal ion binding; nucleotide binding. Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; adenosine biosynthetic process; negative regulation of inflammatory response; purine nucleotide catabolic process; DNA metabolic process; purine base metabolic process; AMP catabolic process. Disease: Calcification Of Joints And Arteries

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage