Mouse Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit | MyBioSource MBS908381 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

catalog :

MBS908381

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS908381

products type :

ELISA Kit

products full name :

Mouse Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

products short name :

prostaglandin D2 synthase 21kDa (brain)

products name syn :

Mouse Prostaglandin-H2 D-isomerase (PTGDS) ELISA kit; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla; prostaglandin D2 synthase 21kDa (brain)

other names :

prostaglandin-H2 D-isomerase; Prostaglandin-H2 D-isomerase; prostaglandin-H2 D-isomerase; PGD2 synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin-D synthase; prostaglandin D2 synthase (21 kDa, brain); prostaglandin D2 synthase (brain); Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; L-PGDS; PGD2 synthase; PGDS2

products gene name :

PTGDS

other gene names :

Ptgds; Ptgds; PGD2; PGDS; 21kDa; PGDS2; Ptgs3; L-PGDS; L-PGDS; PGD2 synthase; PGDS2

uniprot entry name :

PTGDS_MOUSE

reactivity :

Mouse

sequence length :

189

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse PTGDS. No significant cross-reactivity or interference between mouse PTGDS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.7 ng/ml -300 ng/ml. Sensitivity: The minimum detectable dose of mouse PTGDS is typically less than 1.2 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTGDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGDS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGDS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

119226208

ncbi acc num :

NP_032989.2

ncbi gb acc num :

NM_008963.2

uniprot acc num :

O09114

ncbi mol weight :

21,066 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Arachidonic Acid Metabolism Pathway (522975); Arachidonic Acid Metabolism Via COX (Cyclooxygenase) Pathway (522976); Eicosanoid Synthesis Pathway (198357); Folic Acid Network Pathway (198383); Prostaglandin Synthesis And Regulation Pathway (198286); Biosynthesis Of Prostaglandins Pathway (142890); Selenium Pathway (198359)

uniprot summary :

PTGDS: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Belongs to the calycin superfamily. Lipocalin family. Protein type: Endoplasmic reticulum; EC 5.3.99.2; Secreted; Lipid Metabolism - arachidonic acid; Isomerase; Secreted, signal peptide. Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; membrane; endoplasmic reticulum; cytoplasm; extracellular region; nucleus. Molecular Function: retinoid binding; prostaglandin-D synthase activity; transporter activity; isomerase activity; fatty acid binding. Biological Process: transport; lipid metabolic process; fatty acid metabolic process; prostaglandin biosynthetic process; fatty acid biosynthetic process; prostaglandin metabolic process; regulation of circadian sleep/wake cycle, sleep

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage