catalog number :
MBS908302
products type :
ELISA Kit
products full name :
Human Leucine-rich repeats and immunoglobulin-like domains protein 1, LRIG1 ELISA Kit
products short name :
leucine-rich repeats and immunoglobulin-like domains 1
products name syn :
Human Leucine-rich repeats and immunoglobulin-like domains protein 1 (LRIG1) ELISA kit; DKFZp586O1624; LIG-1; LIG1; leucine-rich repeat protein LRIG1; ortholog of mouse integral membrane glycoprotein LIG-1; leucine-rich repeats and immunoglobulin-like domains 1
other names :
leucine-rich repeats and immunoglobulin-like domains protein 1; Leucine-rich repeats and immunoglobulin-like domains protein 1; leucine-rich repeats and immunoglobulin-like domains protein 1; leucine-rich repeat protein LRIG1; ortholog of mouse integral membrane glycoprotein LIG-1; leucine-rich repeats and immunoglobulin-like domains 1
products gene name :
LRIG1
other gene names :
LRIG1; LRIG1; LIG1; LIG-1; LIG1; LIG-1
uniprot entry name :
LRIG1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human LRIG1. No significant cross-reactivity or interference between Human LRIG1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LRIG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRIG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRIG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRIG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_056356.2
ncbi gb acc num :
NM_015541.2
ncbi mol weight :
119,113 Da
ncbi pathways :
Disease Pathway (530764); ErbB4 Signaling Events Pathway (137991); Internalization Of ErbB1 Pathway (138013); Signal Transduction Pathway (477114); Signaling By EGFR Pathway (106337); Signaling By EGFR In Cancer Pathway (530768)
uniprot summary :
LRIG1: a singhle-pass type I membrane protein that acts as a feedback negative regulator of signaling by receptor tyrosine kinases. Regulates cadherin-dependent contact inhibition. Interacts with EGFR, HER2, -3, and -4. Two isoforms of the human protein are produced by alternative splicing. Protein type: Protein kinase, regulatory subunit; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3p14. Cellular Component: integral to membrane. Biological Process: sensory perception of sound; otolith morphogenesis
size4 :
10x96-Strip-Wells