Human Collagen alpha-1 (VII) chain, COL7A1 ELISA Kit | MyBioSource MBS908240 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Collagen alpha-1 (VII) chain, COL7A1 ELISA Kit

catalog :

MBS908240

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS908240

products type :

ELISA Kit

products full name :

Human Collagen alpha-1 (VII) chain, COL7A1 ELISA Kit

products short name :

collagen, type VII, alpha 1

products name syn :

Human Collagen alpha-1 (VII) chain (COL7A1) ELISA kit; EBD1; EBDCT; EBR1; LC collagen; alpha 1 type VII collagen; collagen VII; alpha-1 polypeptide; long chain collagen; collagen; type VII; alpha 1

other names :

collagen alpha-1(VII) chain; Collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; collagen alpha-1(VII) chain; LC collagen; long-chain collagen; collagen VII, alpha-1 polypeptide; collagen, type VII, alpha 1; Long-chain collagen; LC collagen

products gene name :

COL7A1

other gene names :

COL7A1; COL7A1; EBD1; EBR1; EBDCT; LC collagen

uniprot entry name :

CO7A1_HUMAN

reactivity :

Human

sequence length :

2944

specificity :

This assay has high sensitivity and excellent specificity for detection of Human COL7A1. No significant cross-reactivity or interference between Human COL7A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Cell Adhesion. Protein Biological Process 3: Cell adhesion

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for COL7A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any COL7A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for COL7A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of COL7A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4502961

ncbi acc num :

NP_000085.1

ncbi gb acc num :

NM_000094.3

uniprot acc num :

Q02388

ncbi mol weight :

295,220 Da

ncbi pathways :

Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Formation Pathway (645288); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes the alpha chain of type VII collagen. The type VII collagen fibril, composed of three identical alpha collagen chains, is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in this gene are associated with all forms of dystrophic epidermolysis bullosa. In the absence of mutations, however, an acquired form of this disease can result from an autoimmune response made to type VII collagen. [provided by RefSeq, Jul 2008]

uniprot summary :

COL7A1: the alpha chain of type VII collagen, an extra-cellular basement membrane protein restricted to the zone beneath stratified squamous epithelia. Type VII collagen fibrils are composed of three identical alpha collagen chains. Forms anchoring fibrils, which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen. Dystrophic epidermolysis bullosa can result from defects in this protein or an autoimmune response made to type VII collagen. Homotrimer. Interacts with MIA3, facilitating its loading into transport carriers and subsequent secretion. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Extracellular matrix; Inhibitor. Chromosomal Location of Human Ortholog: 3p21.1. Cellular Component: extracellular matrix; extracellular space; collagen type VII; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: serine-type endopeptidase inhibitor activity; identical protein binding; protein binding. Biological Process: extracellular matrix disassembly; collagen catabolic process; extracellular matrix organization and biogenesis; epidermis development; cell adhesion. Disease: Transient Bullous Dermolysis Of The Newborn; Epidermolysis Bullosa Dystrophica, Autosomal Dominant; Nail Disorder, Nonsyndromic Congenital, 8; Epidermolysis Bullosa Dystrophica, Autosomal Recessive; Epidermolysis Bullosa Dystrophica, Pretibial; Epidermolysis Bullosa Pruriginosa; Epidermolysis Bullosa With Congenital Localized Absence Of Skin And Deformity Of Nails

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage